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13 March 2024 Fluorescence frequency-response imaging (F-FRI) for nondestructive tissue assessment
Javier A. Jo
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Proceedings Volume PC12862, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications XV; PC1286203 (2024) https://doi.org/10.1117/12.3009510
Event: SPIE BiOS, 2024, San Francisco, California, United States
Abstract
We present a novel, cost-effective, versatile, and practical fluorescence imaging modality, termed Fluorescence Frequency-Response Imaging (F-FRI), capable of imaging the frequency response of the time-resolved fluorescence emission excited at multiple wavelengths and measured at multiple spectral emission bands. Rigorous validation experiments have demonstrated the capabilities of F-FRI to accurately measure fluorescence frequency-response of fluorophores with lifetimes in the range of ~0.4-12 ns. This novel F-FRI imaging modality has been successfully translated for label-free metabolic endoscopic imaging of epithelial oral mucosa, and for label-free biochemical wide-field imaging of resected skin tumors during Mohs surgery.
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Javier A. Jo "Fluorescence frequency-response imaging (F-FRI) for nondestructive tissue assessment", Proc. SPIE PC12862, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications XV, PC1286203 (13 March 2024); https://doi.org/10.1117/12.3009510
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KEYWORDS
Fluorescence
Fluorescence imaging
Tissues
Modulation frequency
Nondestructive evaluation
Field programmable gate arrays
Modulation
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