This PDF file contains the front matter associated with SPIE Proceedings Volume 11921, including the Title Page, Copyright information, and Table of Contents.

The resolution of nuclear imaging systems is very limited. To improve the resolution, a targeted nanoparticles gamma imaging modality based on variable and dynamic pinhole array system is proposed.

We present a technique to capture high spatial resolution, multispectral, and time-resolved fluorescence images by combining both single-pixel and data fusion imaging techniques. The resultant 4D hypercube can be used to identify fluorophore species.

Polarimetric second harmonic generation (SHG) microscopy study of collagen in cancerous and normal tissues showed the differences in SHG intensity, susceptibility ratio R and fiber orientation distribution suggesting a modified collagen structure.

Pre-resonance Stimulated Raman Scattering (SRS) provides enhanced sensitivity up to micromolar region on top of the inherited Raman specificity. We demonstrate the application of our microscope on solutions of antibiotics and treated yeast cells.

Stimulated Brillouin scattering is here discussed, and new optimization strategies are presented for obtaining shot-noise limited Brillouin spectra in significantly shorter acquisition times, with high signal-to-noise ratio and measurement precision.

We are introducing a proof-of concept method to estimate scleral mechanical properties from air-puff deformation imaging using optical coherence tomography (OCT), customized surface segmentation methods, and 3D finite element analysis on porcine eyes.

Quantitative elastography is performed using noise-correlation on full-field images acquired using digital holography. Experimental results in isotropic and anisotropic polymer samples are presented as well as stiffness images on biological tissues.

A new vibrography approach based on OCT is suggested for the measurement of corneal oscillations during external harmonic excitation avoiding the need of synchronization and thus making corneal vibrography accessible with standard OCT equipment.

A novel, interferometry-based approach to micropipette aspiration overcomes the spatial resolution limit of current methods, simplifies data retrieval, and enables the study of the dynamic mechanical behavior of biomembranes at the nanoscale.

A recently built set-up based on correlative Brillouin and Raman micro-spectroscopies, provides the simultaneous characterization of viscoelastic properties and chemical composition with subcellular resolution. Results obtained in cells and tissues show its diagnostic potential.

Using attenuated total reflection in the terahertz domain, we studied the dynamics of MDCK cells after permeabilization by saponin molecules. We developed an analytical model taking into account molecules diffusion, cell geometry, and pore dynamics.

Raman-Trapping-Microscopy integrates Nobel prize technologies--Raman and Optical Trapping--into a microscope making it available for biomedical use. Cells, bacteria, exosomes or viruses are analyzed reflecting their natural features and behavior.

We have developed a low-cost (material cost < $2,000) chromatic confocal endomicroscope capable of cross-sectional imaging of the tissue at lateral and axial resolution of 1.55μm and 9.5μm, respectively.

We report a single plane illumination microscope dedicated to image “Organ-on-chip”-like biostructures in microfluidic systems. By allowing 3D fluorescence imaging inside the chip, it will permit to follow in time sample’s development at cellular scale.

We explore the use of a holographic system using a spatial light modulator combined with calcium imaging to excite ChR2 expressing cardiomyocytes in order to simultaneously probe and visualize the excitability of a cardiac syncytium.

In this proceeding, we characterize the anisotropic properties of skin that strongly scatter propagating light. We conduct the measurement by probing the optical near field from the sample side with focused surface plasmon.

Discoid Lupus Erythematosus is a dermatological disease difficult to diagnose. By using Raman spectroscopy and MALDI mass spectrometry a shift in lipid biochemistry has been identified relative to healthy tissue, that can improve early detection.

We compared the bacterial inactivating effect of continuous light and pulsed light irradiation to develop a novel technology for safety transfusion. This experiment demonstrated that pulsed light achieved higher inactivation than continuous light.

Live lung cancer cells in vitro were exposed to selected medicaments with putative anti-metastatic potential and examined by time-lapse hiQPI, providing simultaneous measurements of the effect on cell growth and motility with unprecedented accuracy.

This work illustrates how tumor proportional score is estimated using object detection method YOLO and compared with a pathologist's calculation. Results show deep learning can achieve good results and be used on clinical applications.

To stimulate neural ensemble selectively at high resolution, we propose non-convex optimization with spherical aberration compensation algorithm for two-photon optogenetics, which achieves high excitation efficiency and precise axial positioning.

The results of quantitative, label-free measurements of SHSY-5Y cells under Low-Level Laser Therapy treatment are presented. The time-lapse investigations performed by means of digital holographic microscopy are focused on dry mass density changes after light exposure.

We fabricated SERS-based biosensors using the GLAD technique. The enhancement in Raman signal was investigated using 0.01M Rhodamine 6G solution, and it was employed to detect and identify biological pathogens like E-Coli bacteria.

THz-TDS measurements of biological cells with minimal sample preparations reveal characteristic time of cellular breakdown due to dehydration. Using this scheme, species-level discrimination based on the cell biomechanical properties is proposed.

Pump-probe microscopy can be utilized to image laser-induced acoustic waves, which carry information about elastic properties. This paper shows how the pulse number and energy affects the imaging quality of waves in water and glass.

We report experiments on photodynamic treatment of HeLa cells with 5-ALA. Intracellular localization of generated PpIX was determined and combinations of treatment parameters providing major pathways of cell death were obtained using digital holographic tomography.

3D-models based on patient-specific induced pluripotent stem cells are widely used for biomedical research. Thus, non-invasive and continuous comprehensive description of these of 3D-models using FLIM and multiphoton microscopy requires.

The optical transfer function of different scattering media was approximated with a Neural Network. The chosen U-architecture allowed for reconstructing the initial images. It was found that pretrained networks could be adapted for similar tasks.