Label-free three dimensional optical coherence tomography (OCT-) based imaging method for drug response evaluation of human derived tumor spheroids

Ibrahim G. Abd El-Sadek; Arata Miyazawa; Tomoko Mori; Larina Shen; Shuichi Makita; Pradipta Mukherjee; Satoshi Matsusaka; Yoshiaki Yasuno

doi:10.1117/12.2608156

2 March 2022 Label-free three dimensional optical coherence tomography (OCT-) based imaging method for drug response evaluation of human derived tumor spheroids

Ibrahim G. Abd El-Sadek, Arata Miyazawa, Tomoko Mori, Larina Shen, Shuichi Makita, Pradipta Mukherjee, Satoshi Matsusaka, Yoshiaki Yasuno

Author Affiliations +

Proceedings Volume PC11972, Label-free Biomedical Imaging and Sensing (LBIS) 2022; PC119720N (2022) https://doi.org/10.1117/12.2608156
Event: SPIE BiOS, 2022, San Francisco, California, United States

Abstract

We present a non-invasive (label-free) and 3-D method for anti-cancer drug response evaluation of human originated tumor spheroids. Our method is a combination of an OCT microscope and a statistical analysis framework of the OCT signal temporal fluctuations induced by the intra-cellular motions. This method allows the visualization of the 3-D metabolic activity of tumor spheroids and its alteration induced by the drug applications. Two human originated tumor spheroid’s drug responses were evaluated including breast adenocarcinoma (MCF7 cell-line) and colorectal carcinoma (HT-29 cell-line). The drug response was not only visualized as an images, but also quantitative analysis has been performed.

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Ibrahim G. Abd El-Sadek, Arata Miyazawa, Tomoko Mori, Larina Shen, Shuichi Makita, Pradipta Mukherjee, Satoshi Matsusaka, and Yoshiaki Yasuno "Label-free three dimensional optical coherence tomography (OCT-) based imaging method for drug response evaluation of human derived tumor spheroids", Proc. SPIE PC11972, Label-free Biomedical Imaging and Sensing (LBIS) 2022, PC119720N (2 March 2022); https://doi.org/10.1117/12.2608156

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