Comprehensive histology of fresh tumor-resection margins with rapid open-top light-sheet (OTLS) microscopy

Gan Gao; Dominie Miyasato; Lindsey A. Barner; Lawrence D. True; Jeffrey Houlton; Jonathan T. C. Liu

doi:10.1117/12.2650687

17 March 2023 Comprehensive histology of fresh tumor-resection margins with rapid open-top light-sheet (OTLS) microscopy

Gan Gao, Dominie Miyasato, Lindsey A. Barner, Lawrence D. True, Jeffrey Houlton, Jonathan T. C. Liu

Author Affiliations +

Proceedings Volume PC12363, Multiscale Imaging and Spectroscopy IV; PC123630E (2023) https://doi.org/10.1117/12.2650687
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

Margin status directly correlates with patient survival in many types of cancer. To improve the identification of positive margins, we report a rapid surgical guidance workflow including tissue staining, open-top light-sheet (OTLS) microscopy imaging, and post-processing to generate en face histologic images of fresh tissue surfaces. Compared with conventional frozen section analysis (FSA) in which a few vertical sections of tissue are sampled, our technology could comprehensively image large margin surfaces in a non-destructive manner and achieve superior image quality. We provide examples showing that the image quality generated by our rapid surface-histology method approaches that of gold-standard archival histology.

Conference Presentation

Citation Download Citation

Gan Gao, Dominie Miyasato, Lindsey A. Barner, Lawrence D. True, Jeffrey Houlton, and Jonathan T. C. Liu "Comprehensive histology of fresh tumor-resection margins with rapid open-top light-sheet (OTLS) microscopy", Proc. SPIE PC12363, Multiscale Imaging and Spectroscopy IV, PC123630E (17 March 2023); https://doi.org/10.1117/12.2650687

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