13 March 2024Comparison of the effectiveness of two different light spectra in 5-ALA-based antimicrobial photodynamic therapy of orthopaedic implant-relevant biofilms
Logan M. Bateman,1 Valentin V. Demidovhttps://orcid.org/0000-0002-0960-3858,2,3 Natalia Demidova,4 Jason R. Gunn,2 Scott J. Sottosanti,2 Eric R. Henderson,2,5,6 Ida L. Gitajn,2,6 Jonathan T. Elliot2,5,6
1Thayer School of Engineering at Dartmouth, Dartmouth College (United States) 2Dartmouth-Hitchcock Medical Ctr. (United States) 3Geisel School of Medicine, Dartmouth College (United States) 4Univ. of Toronto (Canada) 5Thayer School of Engineering at Dartmouth (United States) 6Geisel School of Medicine (United States)
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Bacterial biofilms are a pervasive issue in orthopaedic surgery causing up to 80% of infections. Antimicrobial photodynamic therapy (aPDT) is a proposed technique for eradication of biofilms, clinical translation requires optimization of treatment parameters. This study assessed the effectiveness of three light spectra in activating photosensitizing porphyrins to kill a dual species biofilm of E. Coli and E. faecalis grown in a microfluidic device. Blue-red, amber, or blue-amber-red light sources were compared at either 30J/cm2 or 60J/cm2 doses given to activate endogenously produced porphyrins after one hour incubation with 10 or 20% 5-ALA in a saline solution. Changes in biomass 24 hours after treatment were measured using confocal microscopy and OCT to determine treatment effectiveness.
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Logan M. Bateman, Valentin V. Demidov, Natalia Demidova, Jason R. Gunn, Scott J. Sottosanti, Eric R. Henderson, Ida L. Gitajn, Jonathan T. Elliot, "Comparison of the effectiveness of two different light spectra in 5-ALA-based antimicrobial photodynamic therapy of orthopaedic implant-relevant biofilms," Proc. SPIE PC12822, Photonic Diagnosis, Monitoring, Prevention, and Treatment of Infections and Inflammatory Diseases 2024, PC128220G (13 March 2024); https://doi.org/10.1117/12.3003128