Presentation
13 March 2024 Unravelling molecular and biophysical fingerprints from single particles using digital holographic microscopy
Jaime Ortega Arroyo
Author Affiliations +
Proceedings Volume PC12852, Quantitative Phase Imaging X; PC128520Q (2024) https://doi.org/10.1117/12.3002986
Event: SPIE BiOS, 2024, San Francisco, California, United States
Abstract
Simultaneously delivering specificity, high throughput, and sensitivity has been one of the long-sought goals in label-free all-optical approaches. Particularly in the context of sensing, accurate characterisation of heterogeneous nanoparticle populations demands methods that comply with these three key properties at the single particle level. Nevertheless, this poses a significant challenge to date simply because the smaller the size of a particle, the greater the demand on sensitivity, which usually is paid in the currency of throughput. Similarly, the greater the number of markers used to provide specificity, the lower the throughput. Here, I will introduce a series of optofluidic platforms developed in the group that uniquely fingerprint heterogeneous nanoparticle suspensions by integrating digital holographic microscopy with microfluidics and surface chemistry.
Conference Presentation
© (2024) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Jaime Ortega Arroyo "Unravelling molecular and biophysical fingerprints from single particles using digital holographic microscopy", Proc. SPIE PC12852, Quantitative Phase Imaging X, PC128520Q (13 March 2024); https://doi.org/10.1117/12.3002986
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KEYWORDS
Digital holography

Particles

Holography

Microscopy

Nanoparticles

3D tracking

Microfluidics

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