Laser-induced immune modulation inhibits tumor growth in vivo (Conference Presentation)

Giulia Ottaviani; Valentina Martinelli; Katia Rupel; Nicoletta Caronni; Asma Naseem; Lorenzo Zandonà; Giuseppe Perinetti; Margherita Gobbo; Roberto Di Lenarda; Rossana Bussani; Federica Benvenuti; Mauro Giacca; Matteo Biasotto; Serena Zacchigna

doi:10.1117/12.2257477

19 April 2017 Laser-induced immune modulation inhibits tumor growth in vivo (Conference Presentation)

Giulia Ottaviani, Valentina Martinelli, Katia Rupel, Nicoletta Caronni, Asma Naseem, Lorenzo Zandonà, Giuseppe Perinetti, Margherita Gobbo, Roberto Di Lenarda, Rossana Bussani, Federica Benvenuti, Mauro Giacca, Matteo Biasotto, Serena Zacchigna

Author Affiliations +

Proceedings Volume 10048, Mechanisms of Photobiomodulation Therapy XII; 100480F (2017) https://doi.org/10.1117/12.2257477
Event: SPIE BiOS, 2017, San Francisco, California, United States

Abstract

Photobiomodulation stands as a recommended therapy for oral mucositis induced by oncological therapies. However, its mechanisms of action and, more importantly, its safety in cancer patients, are still unclear. We assessed cancer cell metabolism and proliferation in vitro and in vivo after exposure to different laser protocols. We exploited both ectopic melanoma and a more physiological oral carcinogenesis mouse model, followed by molecular, histological and immunohistochemical characterization. Laser irradiation resulted in a slightly increase in cell metabolism and proliferation in vitro, albeit each protocol exerted a difference response. Of notice, in vivo laser light reduced tumour growth and invasiveness, indicating e beneficial effect on tumor microenvironment. Laser-treated tumors were surrounded and infiltrated by immune cells, mainly lymphocytes and dendritic cells, paralleled by an enhanced secretion of type I interferons. In contrast, the number of pro-angiogenic macrophages was reduced in response to laser irradiation, with consequent normalization of the tumor vasculature. Based on these finding we have also started exploring the effect of photobiomodulation on lymphocyte response in an experimental model of vaccination. Preliminary data indicate that laser light induced antigen-specific CD8+ and CD4+ T cell responses. In conclusion, our data point toward photobiomodulation as an effective strategy to boost the immune response in vivo, with relevant, therapeutic activities in both cancer and vaccination experimental models. These results support the safe use of laser light on cancer patients and open the way to innovative therapeutic opportunities.

Conference Presentation

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Giulia Ottaviani, Valentina Martinelli, Katia Rupel, Nicoletta Caronni, Asma Naseem, Lorenzo Zandonà, Giuseppe Perinetti, Margherita Gobbo, Roberto Di Lenarda, Rossana Bussani, Federica Benvenuti, Mauro Giacca, Matteo Biasotto, and Serena Zacchigna "Laser-induced immune modulation inhibits tumor growth in vivo (Conference Presentation)", Proc. SPIE 10048, Mechanisms of Photobiomodulation Therapy XII, 100480F (19 April 2017); https://doi.org/10.1117/12.2257477

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KEYWORDS

In vivo imaging

Tumors

Cancer

Laser therapeutics

Data modeling

In vitro testing

Laser irradiation

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