Evaluation of the efficacy of AmPDT of oral microorganisms with photogem associated to red LED (λ640nm±5nm): in vitro

Gustavo M. Pires Santos; Susana C. P. S. de Oliveira D.D.S.; Juliana S. de C. Monteiro; Aparecida Maria C. Marques D.D.S.; Vanderlei S. Bagnato; Antônio L. B. Pinheiro

doi:10.1117/12.2251181

17 February 2017 Evaluation of the efficacy of AmPDT of oral microorganisms with Photogem associated to red LED (λ640ηm±5ηm): in vitro

Gustavo M. Pires Santos, Susana C. P. S. de Oliveira D.D.S., Juliana S. de C. Monteiro, Aparecida Maria C. Marques D.D.S., Vanderlei S. Bagnato, Antônio L. B. Pinheiro

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Proceedings Volume 10048, Mechanisms of Photobiomodulation Therapy XII; 100480O (2017) https://doi.org/10.1117/12.2251181
Event: SPIE BiOS, 2017, San Francisco, California, United States

Abstract

The aim of this study is to evaluate the Antimicrobial Photodynamic Therapy (AmPDT) of microorganisms mediated by red LED (λ640 ± 5 ηm, 120 mW, spot of 0.785 cm²) associated with Photogem. Microorganisms of the posterior dorsal region of the tongue and oral mucosa were collected and inoculated in 8 mL of TSB medium overnight followed by inverse homogenization. Culture plates with 24 wells were used for the irradiated and non-irradiated species. Each well received 400 μL of the suspension containing the microorganisms. In eight wells no Photogem was used and they were the irradiated and non-irradiated controls. The remain of the wells had Photogem applied with a pre-irradiation time of 5 min in concentrations of 40, 20, 10, 5 e 2.5 μg/mL. LED was applied for 5 min and 45 sec (50 J/cm²). After agitation, 200 μL were withdrawn from each well and colorimetric measurements were immediately taken. Other 200 μL were withdrawn from the wells after 1 hour in bacteriological incubator for a second measurement. The best results found were for 40 μg/mL of Photogem associated with LED irradiation and immediate (36.7%) and for 5 μg/mL with LED irradiation with measurements one hour after incubation (42.8%). This study suggests that antimicrobial photodynamic therapy with Photogem associated to red LED can be a potential mechanism of control of oral microorganisms.

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Gustavo M. Pires Santos, Susana C. P. S. de Oliveira D.D.S., Juliana S. de C. Monteiro, Aparecida Maria C. Marques D.D.S., Vanderlei S. Bagnato, and Antônio L. B. Pinheiro "Evaluation of the efficacy of AmPDT of oral microorganisms with Photogem associated to red LED (λ640ηm±5ηm): in vitro", Proc. SPIE 10048, Mechanisms of Photobiomodulation Therapy XII, 100480O (17 February 2017); https://doi.org/10.1117/12.2251181

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KEYWORDS

Light emitting diodes

Microorganisms

In vitro testing

Photodynamic therapy

Light-emitting diode therapy

Resistance

Dentistry

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