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19 April 2017 Standardization of fluorescence measurements in the UV/vis/NIR/IR: needs for and requirements on calibration tools (Conference Presentation)
Ute Resch-Genger, Jutta Pauli, Katrin Hoffmann, Christian Würth, Thomas Behnke
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Proceedings Volume 10049, Molecular-Guided Surgery: Molecules, Devices, and Applications III; 100490C (2017) https://doi.org/10.1117/12.2255728
Event: SPIE BiOS, 2017, San Francisco, California, United States
Abstract
Photoluminescence techniques are amongst the most widely used tools in the life sciences, with new and exciting applications in medical diagnostics and molecular imaging continuously emerging. Advantages include their comparative ease of use, unique sensitivity, non-invasive character, and potential for multiplexing, remote sensing, and miniaturization. General drawbacks are, however, signals, that contain unwanted wavelength- and polarization contributions from instrument-dependent effects, which are also time-dependent due to aging of instrument-components, and difficulties to measure absolute fluorescence intensities [1]. Moreover, scattering systems require special measurement geometries [2] and the interest in new optical reporters with emission > 1000 nm strategies for reliable measurements in the second diagnostic for the comparison of material performance and the rational design of new fluorophores with improved properties [3]. Here, we present strategies to versatile method-adaptable liquid and solid fluorescence standards for different fluorescence parameters including traceable instrument calibration procedures and the design of integrating sphere setups for the absolute measurement of emission spectra and quantum yields in the wavelength region of 350 to 1600 nm [4,5]. Examples are multi-emitter glasses, spectral fluorescence standards, and quantum yield standards for the UV/vis/NIR.
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Ute Resch-Genger, Jutta Pauli, Katrin Hoffmann, Christian Würth, and Thomas Behnke "Standardization of fluorescence measurements in the UV/vis/NIR/IR: needs for and requirements on calibration tools (Conference Presentation)", Proc. SPIE 10049, Molecular-Guided Surgery: Molecules, Devices, and Applications III, 100490C (19 April 2017); https://doi.org/10.1117/12.2255728
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KEYWORDS
Luminescence
Calibration
Quantum efficiency
Life sciences
Medical diagnostics
Molecular imaging
Multiplexing
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