3D Monte Carlo model with direct photon flux recording for optimal optogenetic light delivery

Younghoon Shin; Dongmok Kim; Jihoon Lee; Hyuk-Sang Kwon

doi:10.1117/12.2250679

8 February 2017 3D Monte Carlo model with direct photon flux recording for optimal optogenetic light delivery

Younghoon Shin, Dongmok Kim, Jihoon Lee, Hyuk-Sang Kwon

Proceedings Volume 10052, Optogenetics and Optical Manipulation; 100520G (2017) https://doi.org/10.1117/12.2250679
Event: SPIE BiOS, 2017, San Francisco, California, United States

Abstract

Configuring the light power emitted from the optical fiber is an essential first step in planning in-vivo optogenetic experiments. However, diffusion theory, which was adopted for optogenetic research, precluded accurate estimates of light intensity in the semi-diffusive region where the primary locus of the stimulation is located. We present a 3D Monte Carlo model that provides an accurate and direct solution for light distribution in this region. Our method directly records the photon trajectory in the separate volumetric grid planes for the near-source recording efficiency gain, and it incorporates a 3D brain mesh to support both homogeneous and heterogeneous brain tissue. We investigated the light emitted from optical fibers in brain tissue in 3D, and we applied the results to design optimal light delivery parameters for precise optogenetic manipulation by considering the fiber output power, wavelength, fiber-to-target distance, and the area of neural tissue activation.

Conference Presentation

Citation Download Citation

Younghoon Shin, Dongmok Kim, Jihoon Lee, and Hyuk-Sang Kwon "3D Monte Carlo model with direct photon flux recording for optimal optogenetic light delivery", Proc. SPIE 10052, Optogenetics and Optical Manipulation, 100520G (8 February 2017); https://doi.org/10.1117/12.2250679

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