Rapid structuring of proteins on filter paper using lithography

Tobias M. Nargang; Frederik Kotz; Nico Keller; Dorothea Helmer; Bastian E. Rapp

doi:10.1117/12.2252116

28 February 2017 Rapid structuring of proteins on filter paper using lithography

Tobias M. Nargang, Frederik Kotz, Nico Keller, Dorothea Helmer, Bastian E. Rapp

Proceedings Volume 10061, Microfluidics, BioMEMS, and Medical Microsystems XV; 1006104 (2017) https://doi.org/10.1117/12.2252116
Event: SPIE BiOS, 2017, San Francisco, California, United States

Abstract

Microfluidic paper based analytical devices (μPADs) are simple and cost efficient and can be used everywhere without the need for a high standard laboratory for obtaining a readout. These devices are thus especially suited for the developing world or crisis regions. To fabricate a bioanalytical test, certain biomolecules like proteins or antibodies have to be attached to paper strips. Common immobilization methods often rely on non-covalent, unoriented attachment which leads to reduced bioactivity of the immobilized species. Specific Immobilization of biomolecules on surfaces still poses a great challenge to biochemical research and applications.

We propose a method for the specific immobilization of biomolecules on functionalized filter paper using a maskless projection lithography setup. The paper was functionalized either by applying an adhesive protein coating or by covalent attachment of methacrylate groups. Fluorescently labelled biomolecules were attached by exploiting the formation of radical species upon bleaching of the fluorophore. A custom made maskless photo-lithography setup and a low cost approach were used to produce microscale biomolecule greyscale patterns. Protein patterns were visualized by fluorescence, enzyme patterns were tested for bioactivity by substrate conversion with colorimetric readout.

This method enables the creation of complex, highly specific bioactive protein patterns and greatly facilitates the production of μPADs.

Citation Download Citation

Tobias M. Nargang, Frederik Kotz, Nico Keller, Dorothea Helmer, and Bastian E. Rapp "Rapid structuring of proteins on filter paper using lithography", Proc. SPIE 10061, Microfluidics, BioMEMS, and Medical Microsystems XV, 1006104 (28 February 2017); https://doi.org/10.1117/12.2252116

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
6 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Proteins

Coating

Lithography

Photomasks

Digital micromirror devices

Microfluidics

Nitrogen

Show All Keywords

Keywords/Phrases

Search In:

Publication Years