Correlating measured transient temperature rises with damage rate processes in cultured cells

Michael L. Denton; Amanda J. Tijerina; Cherry C. Gonzalez; B. Giovana Gamboa; Gary D. Noojin; Elharith M. Ahmed; John M. Rickman; Phillip H. Dyer; Benjamin A. Rockwell

doi:10.1117/12.2250916

15 February 2017 Correlating measured transient temperature rises with damage rate processes in cultured cells

Michael L. Denton, Amanda J. Tijerina, Cherry C. Gonzalez, B. Giovana Gamboa, Gary D. Noojin, Elharith M. Ahmed, John M. Rickman, Phillip H. Dyer, Benjamin A. Rockwell

Author Affiliations +

Proceedings Volume 10062, Optical Interactions with Tissue and Cells XXVIII; 100620I (2017) https://doi.org/10.1117/12.2250916
Event: SPIE BiOS, 2017, San Francisco, California, United States

Abstract

Thermal damage rate processes in biological tissues are usually characterized by a kinetics approach. This stems from experimental data that show how the transformation of a specified biological property of cells or biomolecule (plating efficiency for viability, change in birefringence, tensile strength, etc.) is dependent upon both time and temperature. Here, two disparate approaches were used to study thermal damage rate processes in cultured retinal pigment epithelial cells. Laser exposure (photothermal) parameters included 2-μm laser exposure of non-pigmented cells and 532-nm exposures of cells possessing a variety of melanosome particle densities. Photothermal experiments used a mid-IR camera to record temperature histories with spatial resolution of about 8 μm, while fluorescence microscopy of the cell monolayers identified threshold damage at the boundary between live and dead cells. Photothermal exposure durations ranged from 0.05-20 s, and the effects of varying ambient temperature were investigated. Temperature during heat transfer using a water-jacketed cuvette was recorded with a fast microthermister, while damage and viability of the suspended cells were determined as percentages. Exposure durations for the heat transfer experiments ranged from 50- 60 s. Empirically-determined kinetic parameters for the two heating methods were compared with each other, and with values found in the literature.

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Michael L. Denton, Amanda J. Tijerina, Cherry C. Gonzalez, B. Giovana Gamboa, Gary D. Noojin, Elharith M. Ahmed, John M. Rickman, Phillip H. Dyer, and Benjamin A. Rockwell "Correlating measured transient temperature rises with damage rate processes in cultured cells", Proc. SPIE 10062, Optical Interactions with Tissue and Cells XXVIII, 100620I (15 February 2017); https://doi.org/10.1117/12.2250916

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