Photoacoustic measurements of red blood cell oxygen saturation in blood bags in situ

Ruben N. Pinto; Karan Bagga; Alexandre Douplik; Jason P. Acker; Michael C. Kolios

doi:10.1117/12.2253530

3 March 2017 Photoacoustic measurements of red blood cell oxygen saturation in blood bags in situ

Ruben N. Pinto, Karan Bagga, Alexandre Douplik, Jason P. Acker, Michael C. Kolios

Proceedings Volume 10064, Photons Plus Ultrasound: Imaging and Sensing 2017; 1006440 (2017) https://doi.org/10.1117/12.2253530
Event: SPIE BiOS, 2017, San Francisco, California, United States

Abstract

Red blood cell (RBC) transfusion is a critical component of the health care services. RBCs are stored in blood bags in hypothermic temperatures for a maximum of 6 weeks post donation. During this in vitro storage period, RBCs have been documented to undergo changes in structure and function due to mechanical and biochemical stress. Currently, there are no assessment methods that monitor the quality of RBCs within blood bags stored for transfusion. Conventional assessment methods require the extraction of samples, consequently voiding the sterility of the blood bags and potentially rendering them unfit for transfusions. It is hypothesized that photoacoustic (PA) technology can provide a rapid and non-invasive indication of RBC quality. In this study, a novel PA setup was developed for the acquisition of oxygen saturation (SO₂) of two blood bags in situ. These measurements were taken throughout the lifespan of the blood bags (42 days) and compared against the clinical gold standard method of the blood gas analyzer (BGA). SO₂ values of the blood bags increased monotonically throughout the storage period. A strong correlation between PA SO₂ and BGA SO₂ was found, however, PA values were on average 3.5% lower. Both techniques found the bags to increase by an SO₂ of approximately 20%, and measured very similar rates of SO₂ change. Future work will be focused on determining the cause of discrepancy between SO₂ values acquired from PA versus BGA, as well as establishing links between the measured SO₂ increase and other changes in RBC in situ.

Citation Download Citation

Ruben N. Pinto, Karan Bagga, Alexandre Douplik, Jason P. Acker, and Michael C. Kolios "Photoacoustic measurements of red blood cell oxygen saturation in blood bags in situ", Proc. SPIE 10064, Photons Plus Ultrasound: Imaging and Sensing 2017, 1006440 (3 March 2017); https://doi.org/10.1117/12.2253530

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