Quantitative evaluation of blood flow obstruction in microcirculation with sidestream dark-field images

M. Takahashi; T. Kurata; T. Ohnishi; H. Haneishi

doi:10.1117/12.2253402

16 February 2017 Quantitative evaluation of blood flow obstruction in microcirculation with sidestream dark-field images

M. Takahashi, T. Kurata, T. Ohnishi, H. Haneishi

Author Affiliations +

Proceedings Volume 10068, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XV; 100680A (2017) https://doi.org/10.1117/12.2253402
Event: SPIE BiOS, 2017, San Francisco, California, United States

Abstract

Septic shock induces organ dysfunction by microcirculatory disturbance. Observation and quantification of microcirculation are expected to be effective for the diagnosis of septic shock. Sidestream dark-filed (SDF) imaging is a suitable technique for observation of microcirculation. It can noninvasively visualize red blood cells (RBCs) of microcirculation. We are developing early diagnostic criteria for septic shock from microcirculation SDF images. As an initial study, we use the blood flow velocity estimated from the images as a diagnostic criteria. However, low contrast quality and subject’s movement disturb the blood flow velocity estimation. In this paper, we present a procedure of image processing for a stable estimation of the blood flow velocity. In the procedure, we first perform a robust principal component analysis (RPCA) as a preprocessing. RPCA decomposes a motion picture into a low-rank (L) component and a sparse (S) component. The S component images clearly expresses RBCs flow and is used for the velocity estimation. The temporal change of the intensity profile along the vessel was analyzed by Hough transform to estimate the blood flow velocity is. The proposed procedure was examined with dorsal microcirculation of septic model rats and a sham rat. As a result, the decrease in blood flow velocity of the septic rats after 17 hours was greater than that of the sham. It was also suggested that blood flow velocity might be faster index of septic shock reaction earlier than lactic acid value. These results suggest that the velocity estimation is reasonable for diagnosis of septic shock.

Conference Presentation

Citation Download Citation

M. Takahashi, T. Kurata, T. Ohnishi, and H. Haneishi "Quantitative evaluation of blood flow obstruction in microcirculation with sidestream dark-field images", Proc. SPIE 10068, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XV, 100680A (16 February 2017); https://doi.org/10.1117/12.2253402

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available