Effects of anti-cancer drug doxorubicin on endogenous biomarkers NAD(P)H, FAD and Trp in prostate cancer cells: a FLIM Study

Shagufta Rehman Alam; Horst Wallrabe; Zdenek Svindrych; Kathryn G. Christopher; Dhyan Chandra; Ammasi Periasamy

doi:10.1117/12.2257715

21 February 2017 Effects of anti-cancer drug doxorubicin on endogenous biomarkers NAD(P)H, FAD and Trp in prostate cancer cells: a FLIM Study

Shagufta Rehman Alam, Horst Wallrabe, Zdenek Svindrych, Kathryn G. Christopher, Dhyan Chandra, Ammasi Periasamy

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Proceedings Volume 10069, Multiphoton Microscopy in the Biomedical Sciences XVII; 100691L (2017) https://doi.org/10.1117/12.2257715
Event: SPIE BiOS, 2017, San Francisco, California, United States

Abstract

Fluorescence Lifetime Imaging Microscopy (FLIM) can be used to identify changes in metabolic activity during cancer progression and upon anti-cancer drug treatment. Prostate cancer (PCa) is one of the leading cancers in men in the USA. This research focusses on understanding the lifetime changes of endogenous biomarkers: NAD(P)H, FAD and Trp in LNCaP cells upon treatment with doxorubicin using our 3-channel FLIM approach. The LNCaP cells were treated with doxorubicin for 24hr. Images using FLIM of LNCaP control and treated cells were acquired on Zeiss 780 multiphoton confocal microscope coupled with B and H TCSPC FLIM board. After FLIM data fitting and processing we observed increase in the mean fluorescence lifetime of Trp, NAD(P)H and FAD with doxorubicin treatment. Additionally, we saw reduction in the NAD(P)H/FAD redox ratio with doxorubicin treatment. Our results identify the changes in the lifetime of these endogenous biomarkers and in the cellular redox state as a metabolic response with doxorubicin treatment in prostate cancer cells.

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Shagufta Rehman Alam, Horst Wallrabe, Zdenek Svindrych, Kathryn G. Christopher, Dhyan Chandra, and Ammasi Periasamy "Effects of anti-cancer drug doxorubicin on endogenous biomarkers NAD(P)H, FAD and Trp in prostate cancer cells: a FLIM Study", Proc. SPIE 10069, Multiphoton Microscopy in the Biomedical Sciences XVII, 100691L (21 February 2017); https://doi.org/10.1117/12.2257715

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