Single image sectioning using spatial frequency domain imaging

Zachary R. Hoffman; Charles A. DiMarzio

doi:10.1117/12.2254734

22 March 2017 Single image sectioning using spatial frequency domain imaging

Zachary R. Hoffman, Charles A. DiMarzio

Proceedings Volume 10070, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXIV; 1007014 (2017) https://doi.org/10.1117/12.2254734
Event: SPIE BiOS, 2017, San Francisco, California, United States

Abstract

Structured illumination microscopy (SIM) works well to produce depth information by removing undesired light from out of focus planes within a specimen. However, it generally requires multiple images in order to produce sectioning. We show that, under the right conditions, we can leverage Spatial Frequency Domain Imaging (SFDI) to produce both sectioning and relative depth information about a specimen using only a single image. After processing, we find that the axial resolution is comparable to that of traditional 3 phase SIM. With this technique, we are able to produce real-time videos in-vivo at 21 frames per second. Additionally, no moving parts are needed to produce sectioning at depth.

Citation Download Citation

Zachary R. Hoffman and Charles A. DiMarzio "Single image sectioning using spatial frequency domain imaging", Proc. SPIE 10070, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXIV, 1007014 (22 March 2017); https://doi.org/10.1117/12.2254734

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KEYWORDS

Image processing

Light scattering

Modulation

In vivo imaging

Skin

3D image reconstruction

Microscopy

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