CONFERENCE PROCEEDINGS
Advanced Search
Home > Proceedings > Volume 10073 > Article
Presentation
24 April 2017 Scattering compensation by focus scanning holographic aberration probing (F-SHARP) (Conference Presentation)
Ioannis N. Papadopoulos, Jean-Sébastien Jouhanneau, James Poulet, Benjamin Judkewitz
Author Affiliations +
Proceedings Volume 10073, Adaptive Optics and Wavefront Control for Biological Systems III; 100730O (2017) https://doi.org/10.1117/12.2251430
Event: SPIE BiOS, 2017, San Francisco, California, United States
Abstract
Optical microscopy is an indispensable tool for researchers, allowing them to closely investigate different organisms, revealing new features and phenomena in biomedical research. Although very useful, conventional imaging techniques that rely only on ballistic, unaffected photons to form images inside inhomogeneous media, like biological tissue, are eventually limited up to the diffusion regime of optical propagation where scattering becomes dominant and no ballistic light can be detected. Adaptive optics and nonlinear optimization methods that rely on so called guide stars have been employed to overcome this problem and image deeper inside biological tissue. These techniques attempt to recover the optimal wavefront that will enhance the image quality or that will render a focus spot inside the scattering biological tissue. In order to achieve that, they have to iterate through each correction mode (e.g. each pixel on a wavefront shaper) thus trading off measurement time with wavefront resolution. Here we present a new turbidity suppression approach, termed Focus Scanning Holographic Aberration Probing (F-SHARP or F♯) that allows us to directly measure the amplitude and phase of the scattered light distribution at the focal plane (scattered E-field PSF). Knowledge of the E-field enables rapid correction of both aberration and scattering with a high resolution. We demonstrate the power of F-SHARP by correcting for aberration and scattering and imaging neuronal structures through the larval zebrafish and mouse brain and through thinned mouse skull in vivo.
Conference Presentation
© (2017) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Citation Download Citation
Ioannis N. Papadopoulos, Jean-Sébastien Jouhanneau, James Poulet, and Benjamin Judkewitz "Scattering compensation by focus scanning holographic aberration probing (F-SHARP) (Conference Presentation)", Proc. SPIE 10073, Adaptive Optics and Wavefront Control for Biological Systems III, 100730O (24 April 2017); https://doi.org/10.1117/12.2251430
ACCESS THE FULL ARTICLE
ORGANIZATIONAL
Sign in with credentials provided by your organization.
INSTITUTIONAL
Select your institution to access the SPIE Digital Library.
SELECT YOUR INSTITUTION
PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.
PERSONAL SIGN IN
No SPIE Account? Create one
PURCHASE THIS CONTENT
SUBSCRIBE TO DIGITAL LIBRARY
50 downloads per 1-year subscription
Members: $195
Non-members: $335 ADD TO CART
25 downloads per 1 - year subscription
Members: $145
Non-members: $250 ADD TO CART
PURCHASE SINGLE ARTICLE
Includes PDF, HTML & Video, when available
Members: $17.00
Non-members: $21.00 ADD TO CART
PROCEEDINGS
 PRESENTATION
WATCH
PRESENTATION SAVE TO MY LIBRARY
GET CITATION
Advertisement
Advertisement
KEYWORDS
Light scattering
Scattering
Wavefronts
Holography
Tissue optics
Scattering compensation
Adaptive optics
RELATED CONTENT
Subscribe to Digital Library
Receive Erratum Email Alert
Back to Top