Mucin1 antibody-conjugated dye-doped mesoporous silica nanoparticles for breast cancer detection in vivo

Juan L. Vivero-Escoto; Laura Moore Jeffords; Didier Dréau; Merlis Alvarez-Berrios; Pinku Mukherjee

doi:10.1117/12.2252369

22 February 2017 Mucin1 antibody-conjugated dye-doped mesoporous silica nanoparticles for breast cancer detection in vivo

Juan L. Vivero-Escoto, Laura Moore Jeffords, Didier Dréau, Merlis Alvarez-Berrios, Pinku Mukherjee

Author Affiliations +

Proceedings Volume 10078, Colloidal Nanoparticles for Biomedical Applications XII; 100780B (2017) https://doi.org/10.1117/12.2252369
Event: SPIE BiOS, 2017, San Francisco, California, United States

Abstract

The development of novel methods for tumor detection is a burgeoning area of research. In particular, the use of silica nanoparticles for optical imaging in the near infrared (NIR) represents a valuable tool because their chemical inertness, biocompatibility, and transparency in the ultraviolet-visible and NIR regions of the electromagnetic spectrum. Moreover, silica nanoparticles can be modified with a wide variety of functional groups such as aptamers, small molecules, antibodies and polymers. Here, we report the development of a mucin 1(MUC1)-specific dye-doped NIR emitting mesoporous silica nanoparticles (MUC1-NIR-MSN) platform for the optical detection of breast cancer tissue overexpressing human tumor-associated MUC1. We have characterized the structural properties and the in vitro performance of this system. The MSN-based optical imaging probe is non-cytotoxic and targets efficiently murine mammary epithelial cancer cells overexpressing human MUC1. Finally, the ability of MUC1-NIR-MSN contrast imaging agent to selectively detect breast cancer tumors overexpressing human tumor-associated MUC1 was successfully demonstrated in a transgenic murine mouse model. The NIR imaging experiments on tumor-bearing animals showed specific accumulation of the MSN-based probe in human MUC1-positive tumors and small signal in control tumors. We envision that this MUC1-specific MSN-based optical probe has the potential to greatly aid in screening prospective patients for early breast cancer detection and in monitoring the efficacy of drug therapy.

Conference Presentation

Citation Download Citation

Juan L. Vivero-Escoto, Laura Moore Jeffords, Didier Dréau, Merlis Alvarez-Berrios, and Pinku Mukherjee "Mucin1 antibody-conjugated dye-doped mesoporous silica nanoparticles for breast cancer detection in vivo", Proc. SPIE 10078, Colloidal Nanoparticles for Biomedical Applications XII, 100780B (22 February 2017); https://doi.org/10.1117/12.2252369

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