The intrinsic fluorescence characteristics of tryptophan, tyrosine and phenyloalanine were presented in this paper. The excitation/emission wavelength of tryptophan, tyrosine and phenyloalanine locate at λex/λem=280/350nm, 275nm/303 and 260/280nm respectively. The excitation and emission bands of these bio-fluorophores are quite overlapped within the EEM area whose excitation wavelength/emission wavelength scope is 230-270nm/260-340nm. Using the PARAFAC algorithm, 10 tryptophan, tyrosine and phenyloalanine mixed solutions and three compound amino acids samples were successfully decomposed, the emission profiles, excitation profiles, central wavelengths and the concentration of the three components were retrieved with high precision, and finally, the tyrosine and taurine were detected from 1st and 2nd sample respectively.
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