Implementation of stimulated Raman scattering microscopy for single cell analysis

Annalisa D'Arco; Maria Antonietta Ferrara; Maurizio Indolfi; Vitaliano Tufano; Luigi Sirleto

doi:10.1117/12.2266776

16 May 2017 Implementation of stimulated Raman scattering microscopy for single cell analysis

Annalisa D'Arco, Maria Antonietta Ferrara, Maurizio Indolfi, Vitaliano Tufano, Luigi Sirleto

Proceedings Volume 10228, Nonlinear Optics and Applications X; 102280S (2017) https://doi.org/10.1117/12.2266776
Event: SPIE Optics + Optoelectronics, 2017, Prague, Czech Republic

Abstract

In this work, we present successfully realization of a nonlinear microscope, not purchasable in commerce, based on stimulated Raman scattering. It is obtained by the integration of a femtosecond SRS spectroscopic setup with an inverted research microscope equipped with a scanning unit. Taking account of strength of vibrational contrast of SRS, it provides label-free imaging of single cell analysis. Validation tests on images of polystyrene beads are reported to demonstrate the feasibility of the approach. In order to test the microscope on biological structures, we report and discuss the label-free images of lipid droplets inside fixed adipocyte cells.

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Citation Download Citation

Annalisa D'Arco, Maria Antonietta Ferrara, Maurizio Indolfi, Vitaliano Tufano, and Luigi Sirleto "Implementation of stimulated Raman scattering microscopy for single cell analysis", Proc. SPIE 10228, Nonlinear Optics and Applications X, 102280S (16 May 2017); https://doi.org/10.1117/12.2266776

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