Inkjet-printed selective microfluidic biosensor using CNTs functionalized by cytochrome P450 enzyme

Matic Krivec; Raimund Leitner; Florian Überall; Johannes Hochleitner

doi:10.1117/12.2264095

30 May 2017 Inkjet-printed selective microfluidic biosensor using CNTs functionalized by cytochrome P450 enzyme

Matic Krivec, Raimund Leitner, Florian Überall, Johannes Hochleitner

Proceedings Volume 10247, Bio-MEMS and Medical Microdevices III; 1024707 (2017) https://doi.org/10.1117/12.2264095
Event: SPIE Microtechnologies, 2017, Barcelona, Spain

Abstract

An additive manufacturing concept, consisting of 3D photopolymer printing and Ag nanoparticle printing, was investigated for the construction of a microfluidic biosensor based on immobilized cytochrome P450 enzyme. An acylate-type microfluidic chamber composed of two parts, i.e. chamber-housing and chamber-lid was printed with a polyjet 3D printer. A 3-electrode sensor structure was inkjet-printed on the lid using a combination of Ag and graphene printing. The working electrode was covered with carbon nanotubes by drop-casting and immobilized with cytochrome P450 2D6 enzyme. The microfluidic sensor shows a significant response to a test xenobiotic, i.e. dextromethorphan; the cyclic voltammetrical measurements show a corresponding oxidation peak at 0.4 V with around 5 μM detection limit.

Citation Download Citation

Matic Krivec, Raimund Leitner, Florian Überall, and Johannes Hochleitner "Inkjet-printed selective microfluidic biosensor using CNTs functionalized by cytochrome P450 enzyme", Proc. SPIE 10247, Bio-MEMS and Medical Microdevices III, 1024707 (30 May 2017); https://doi.org/10.1117/12.2264095

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