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A mathematical analysis is presented for the application of dual wavelength excitation and dual wavelength detection for in vivo fluorescence imaging. A dimensionless ratio-image is derived that is independent of optical properties and detection geometry.
H. J. C. M. Sterenborg
"Image processing for fluorescence imaging using dual wavelength excitation and dual wavelength detection", Proc. SPIE 10311, Medical Optical Tomography: Functional Imaging and Monitoring, 1031111 (5 August 1993); https://doi.org/10.1117/12.2283782
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H. J. C. M. Sterenborg, "Image processing for fluorescence imaging using dual wavelength excitation and dual wavelength detection," Proc. SPIE 10311, Medical Optical Tomography: Functional Imaging and Monitoring, 1031111 (5 August 1993); https://doi.org/10.1117/12.2283782