Bovine serum albumin nanoparticles loaded with Photosens photosensitizer for effective photodynamic therapy

Vitaly Khanadeev; Boris Khlebtsov; Gopinath Packirisamy; Nikolai Khlebtsov

doi:10.1117/12.2269159

24 March 2017 Bovine serum albumin nanoparticles loaded with Photosens photosensitizer for effective photodynamic therapy

Vitaly Khanadeev, Boris Khlebtsov, Gopinath Packirisamy, Nikolai Khlebtsov

Author Affiliations +

Proceedings Volume 10336, Saratov Fall Meeting 2016: Optical Technologies in Biophysics and Medicine XVIII; 103360P (2017) https://doi.org/10.1117/12.2269159
Event: Saratov Fall Meeting 2016: Fourth International Symposium on Optics and Biophotonics, 2016, Saratov, Russian Federation

Abstract

Polymeric nanoparticles (NPs) are widely used for drug delivery applications due to high biodegradability, low toxicity and high loading capacity. The focus of this study is the development of photosensitizer Photosens (PS) loaded albumin NPs for efficient photodynamic therapy (PDT). To fabricate PS-loaded bovine serum albumin nanoparticles (BSA-PS NPs), we used a coacervation method with glutaraldehyde followed by passive loading of PS. Successful loading of PS was confirmed by appearance of characteristic peak in absorption spectrum which allows to determine the PS loading in BSA NPs. The synthesized BSA-PS NPs demonstrated low toxicity to HeLa cells at therapeutic concentrations of loaded PS. Compared to free PS solution, the synthesized BSA-PS NPs generated the singlet oxygen more effectively under laser irradiation at 660 nm. In addition, due to presence of various chemical groups on the surface of BSA-PS NPs, they are capable to adsorb on cell surface and accumulate in cells due to cellular uptake mechanisms. Owing to combination of PD and cell uptake advantages, BSA-PS NPs demonstrated higher efficacy of photodynamic damage to cancer cells as compared to free PS at equivalent concentrations. These results suggest that non-targeted BSA-PS NPs with high PD activity and low-fabrication costs of are promising candidates for transfer to PD clinic treatments.

Citation Download Citation

Vitaly Khanadeev, Boris Khlebtsov, Gopinath Packirisamy, and Nikolai Khlebtsov "Bovine serum albumin nanoparticles loaded with Photosens photosensitizer for effective photodynamic therapy", Proc. SPIE 10336, Saratov Fall Meeting 2016: Optical Technologies in Biophysics and Medicine XVIII, 103360P (24 March 2017); https://doi.org/10.1117/12.2269159

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