Stiffness of RBC optical confinement affected by optical clearing

Oleg V. Grishin; Ivan V. Fedosov; Valery V. Tuchin

doi:10.1117/12.2267942

24 March 2017 Stiffness of RBC optical confinement affected by optical clearing

Oleg V. Grishin, Ivan V. Fedosov, Valery V. Tuchin

Proceedings Volume 10336, Saratov Fall Meeting 2016: Optical Technologies in Biophysics and Medicine XVIII; 103360U (2017) https://doi.org/10.1117/12.2267942
Event: Saratov Fall Meeting 2016: Fourth International Symposium on Optics and Biophotonics, 2016, Saratov, Russian Federation

Abstract

In vivo optical trapping is a novel applied direction of an optical manipulation, which enables one to noninvasive measurement of mechanical properties of cells and tissues in living animals directly. But an application area of this direction is limited because strong scattering of many biological tissues. An optical clearing enables one to decrease the scattering and therefore increase a depth of light penetration, decrease a distortion of light beam, improve a resolution in imaging applications. Now novel methods had appeared for a measurement an optical clearing degree at a cellular level. But these methods aren’t applicable in vivo. In this paper we present novel measurement method of estimate of the optical clearing, which are based on a measurement of optical trap stiffness. Our method may be applicable in vivo.

Citation Download Citation

Oleg V. Grishin, Ivan V. Fedosov, and Valery V. Tuchin "Stiffness of RBC optical confinement affected by optical clearing", Proc. SPIE 10336, Saratov Fall Meeting 2016: Optical Technologies in Biophysics and Medicine XVIII, 103360U (24 March 2017); https://doi.org/10.1117/12.2267942

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KEYWORDS

Optical clearing

Optical tweezers

Particles

Molybdenum

In vivo imaging

Microscopes

Tissues

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