Laser speckle contrast imaging of cerebral blood flow of newborn mice at optical clearing

Polina A. Timoshina; Ekaterina M. Zinchenko; Daria K. Tuchina; Madina M. Sagatova; Oxana V. Semyachkina-Glushkovskaya; Valery V. Tuchin

doi:10.1117/12.2268358

24 March 2017 Laser speckle contrast imaging of cerebral blood flow of newborn mice at optical clearing

Polina A. Timoshina, Ekaterina M. Zinchenko, Daria K. Tuchina, Madina M. Sagatova, Oxana V. Semyachkina-Glushkovskaya, Valery V. Tuchin

Author Affiliations +

Proceedings Volume 10336, Saratov Fall Meeting 2016: Optical Technologies in Biophysics and Medicine XVIII; 1033610 (2017) https://doi.org/10.1117/12.2268358
Event: Saratov Fall Meeting 2016: Fourth International Symposium on Optics and Biophotonics, 2016, Saratov, Russian Federation

Abstract

In this work, we consider the use of optical clearing agents to improve imaging quality of the cerebral blood flow of newborn mice. Aqueous 60%-glycerol solution, aqueous 70%-Omnipaque^TM(300) solution and Omnipaque^TM (300) solution in water/DMSO(25%/5%) were selected as the optical clearing agents. Laser speckle contrast imaging (LSCI) was used for imaging of cerebral blood flow in newborn mice brain during topical optical clearing of tissuesin the area of the fontanelle. These results demonstrate the effectiveness of glycerol and Omnipaque solutions as optical clearing agents for investigation of cerebral blood flow in newborn mice without scalp removing and skull thinning.

Citation Download Citation

Polina A. Timoshina, Ekaterina M. Zinchenko, Daria K. Tuchina, Madina M. Sagatova, Oxana V. Semyachkina-Glushkovskaya, and Valery V. Tuchin "Laser speckle contrast imaging of cerebral blood flow of newborn mice at optical clearing", Proc. SPIE 10336, Saratov Fall Meeting 2016: Optical Technologies in Biophysics and Medicine XVIII, 1033610 (24 March 2017); https://doi.org/10.1117/12.2268358

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