Size dependent studies of metal nanoparticles with bio-fluorophores

Ajeetkumar Patil; Steffy Ballary; Sajan D. George; Santhosh Chidangil

doi:10.1117/12.2271289

13 June 2017 Size dependent studies of metal nanoparticles with bio-fluorophores

Ajeetkumar Patil, Steffy Ballary, Sajan D. George, Santhosh Chidangil

Proceedings Volume 10449, Fifth International Conference on Optical and Photonics Engineering; 104493B (2017) https://doi.org/10.1117/12.2271289
Event: Fifth International Conference on Optical and Photonics Engineering, 2017, Singapore, Singapore

Abstract

Interaction of noble metal nanoparticles (NPs) with fluorophores has been an important research area in the field of material science and biomedical field. In the proximity of a metal nanoparticle, there is a quenching or enhancement in the intrinsic fluorescence of the fluorophore . The conditional quenching of the fluorescence can be used for negative sensing whereas enhancement in the fluorescence can be used to gain greater sensitivity and high signal to noise ratio in the molecular sensing/imaging. The current work deals with the systematic studies to understand the fluorescence quenching for few bio-fluorophores (NADH and FAD) when interacted with different sized silver nano-particles of (10nm, 40nm and 100nm). Home assembled Laser Induced Fluorescence (LIF) set-up was used to study the fluorescence quenching of NADH and FAD for different sized silver nanoparticles.

Citation Download Citation

Ajeetkumar Patil, Steffy Ballary, Sajan D. George, and Santhosh Chidangil "Size dependent studies of metal nanoparticles with bio-fluorophores", Proc. SPIE 10449, Fifth International Conference on Optical and Photonics Engineering, 104493B (13 June 2017); https://doi.org/10.1117/12.2271289

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