Redox reactions of cytochrome c in isolated mitochondria exposed to blue or red lasers using resonance Raman spectroscopy

Michael L. Denton; Cherry C. Gonzalez; Gary D. Noojin; Vladislav V. Yakovlev

doi:10.1117/12.2297709

8 February 2018 Redox reactions of cytochrome c in isolated mitochondria exposed to blue or red lasers using resonance Raman spectroscopy

Michael L. Denton, Cherry C. Gonzalez, Gary D. Noojin, Vladislav V. Yakovlev

Author Affiliations +

Proceedings Volume 10477, Mechanisms of Photobiomodulation Therapy XIII; 104770Q (2018) https://doi.org/10.1117/12.2297709
Event: SPIE BiOS, 2018, San Francisco, California, United States

Abstract

Resonance Raman spectroscopy of cytochrome c was used to follow reduction/oxidation (redox) states of isolated mitochondria in response to blue or red laser exposure. Mitochondria were isolated from hTERT-RPE1 cells and were kept in a buffer formulation known to be conducive to electron transport chain (ETC) activity. Using either pyruvate or succinate as substrates for ETC, we found differences in the redox responses of cytochrome c for different exposure laser irradiance and excitation wavelength. We anticipate that the proposed new method will be valuable in the study of metabolic processes in mitochondria in response to low level laser exposure, and thus aid in elucidating the mechanism(s) of photobiomodulation.

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Michael L. Denton, Cherry C. Gonzalez, Gary D. Noojin, and Vladislav V. Yakovlev "Redox reactions of cytochrome c in isolated mitochondria exposed to blue or red lasers using resonance Raman spectroscopy", Proc. SPIE 10477, Mechanisms of Photobiomodulation Therapy XIII, 104770Q (8 February 2018); https://doi.org/10.1117/12.2297709

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