Measurement of shear-induced diffusion of red blood cells using dynamic light scattering-optical coherence tomography

Jianbo Tang; Sefik Evren Erdener; Baoqiang Li; Buyin Fu; Sava Sakadzic; Stefan A. Carp; Jonghwan Lee; David A. Boas

doi:10.1117/12.2290345

12 February 2018 Measurement of shear-induced diffusion of red blood cells using dynamic light scattering-optical coherence tomography

Jianbo Tang, Sefik Evren Erdener, Baoqiang Li, Buyin Fu, Sava Sakadzic, Stefan A. Carp, Jonghwan Lee, David A. Boas

Author Affiliations +

Proceedings Volume 10481, Neural Imaging and Sensing 2018; 104811N (2018) https://doi.org/10.1117/12.2290345
Event: SPIE BiOS, 2018, San Francisco, California, United States

Abstract

Dynamic Light Scattering-Optical Coherence Tomography (DLS-OCT) takes the advantages of using DLS to measure particle flow and diffusion within an OCT resolution-constrained 3D volume, enabling the simultaneous measurements of absolute RBC velocity and diffusion coefficient with high spatial resolution. In this work, we applied DLS-OCT to measure both RBC velocity and the shear-induced diffusion coefficient within penetrating venules of the somatosensory cortex of anesthetized mice. Blood flow laminar profile measurements indicate a blunted laminar flow profile, and the degree of blunting decreases with increasing vessel diameter. The measured shear-induced diffusion coefficient was proportional to the flow shear rate with a magnitude of ~ 0.1 to 0.5 × 10^-6mm² . These results provide important experimental support for the recent theoretical explanation for why DCS is dominantly sensitive to RBC diffusive motion.

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Jianbo Tang, Sefik Evren Erdener, Baoqiang Li, Buyin Fu, Sava Sakadzic, Stefan A. Carp, Jonghwan Lee, and David A. Boas "Measurement of shear-induced diffusion of red blood cells using dynamic light scattering-optical coherence tomography", Proc. SPIE 10481, Neural Imaging and Sensing 2018, 104811N (12 February 2018); https://doi.org/10.1117/12.2290345

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