Optical coherence tomography of lymphatic vessel endothelial hyaluronan receptors in vivo

Peng Si; Debasish Sen; Rebecca Dutta; Siavash Yousefi; Roopa Dalal; Yonatan Winetraub; Orly Liba; Adam de la Zerda

doi:10.1117/12.2282890

14 February 2018 Optical coherence tomography of lymphatic vessel endothelial hyaluronan receptors in vivo

Peng Si, Debasish Sen, Rebecca Dutta, Siavash Yousefi, Roopa Dalal, Yonatan Winetraub, Orly Liba, Adam de la Zerda

Proceedings Volume 10483, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XXII; 1048326 (2018) https://doi.org/10.1117/12.2282890
Event: SPIE BiOS, 2018, San Francisco, California, United States

Abstract

Optical Coherence Tomography (OCT) imaging of living subjects offers millimeters depth of penetration into tissue while maintaining high spatial resolution. However, because most molecular biomarkers do not produce inherent OCT contrast signals, exogenous contrast agents must be employed to achieve molecular imaging. Here we demonstrate that microbeads (μBs) can be used as effective contrast agents to target cellular biomarkers in lymphatic vessels and can be detected by OCT using a phase variance algorithm. We applied this technique to image the molecular dynamics of lymphatic vessel endothelial hyaluronan receptor 1 (LYVE-1) in vivo, which showed significant down-regulation during tissue inflammation.

Citation Download Citation

Peng Si, Debasish Sen, Rebecca Dutta, Siavash Yousefi, Roopa Dalal, Yonatan Winetraub, Orly Liba, and Adam de la Zerda "Optical coherence tomography of lymphatic vessel endothelial hyaluronan receptors in vivo", Proc. SPIE 10483, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XXII, 1048326 (14 February 2018); https://doi.org/10.1117/12.2282890

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available