DAS: A simple, efficient, scalable and DiI-compatible optical clearing method for intact systems

Lingling Chen; Lina Liu; Guiye Li; Ang Liu; Yingchao Li; Xuejuan Hu; Shuangchen Ruan

doi:10.1117/12.2287608

13 February 2018 DAS: A simple, efficient, scalable and DiI-compatible optical clearing method for intact systems

Lingling Chen, Lina Liu, Guiye Li, Ang Liu, Yingchao Li, Xuejuan Hu, Shuangchen Ruan

Proceedings Volume 10493, Dynamics and Fluctuations in Biomedical Photonics XV; 104930Y (2018) https://doi.org/10.1117/12.2287608
Event: SPIE BiOS, 2018, San Francisco, California, United States

Abstract

There is increasing interest in the three-dimensional visualization and quantification of cellular circuits in the brain and therefore optical clearing methods are highly in demand for brain imaging. In particular, clarification without membrane damage is required to image lipophilic tracer-labeled neural tracts. However, previously reported DiI-compatible optical clearing methods are relatively slow and can hinder transparency for imaging. Here, we present DAS, a new, convenient, inexpensive and reproducible aqueous clearing reagent that can efficiently clarify tissues with minimal volume enlargement and reliably preserves emission from fluorescent proteins and lipophilic dyes in membrane integrity preserved tissues.

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Lingling Chen, Lina Liu, Guiye Li, Ang Liu, Yingchao Li, Xuejuan Hu, and Shuangchen Ruan "DAS: A simple, efficient, scalable and DiI-compatible optical clearing method for intact systems", Proc. SPIE 10493, Dynamics and Fluctuations in Biomedical Photonics XV, 104930Y (13 February 2018); https://doi.org/10.1117/12.2287608

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KEYWORDS

Tissues

Brain

Optical clearing

Luminescence

Neuroimaging

Transparency

Optical imaging

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