Label-free counting of circulating cells by in vivo photoacoustic flow cytometry

Quanyu Zhou; Ping Yang; Qiyan Wang; Kai Pang; Hui Zhou; Hao He; Xunbin Wei

doi:10.1117/12.2289216

19 February 2018 Label-free counting of circulating cells by in vivo photoacoustic flow cytometry

Quanyu Zhou, Ping Yang, Qiyan Wang, Kai Pang, Hui Zhou, Hao He, Xunbin Wei

Proceedings Volume 10495, Biophotonics and Immune Responses XIII; 104950T (2018) https://doi.org/10.1117/12.2289216
Event: SPIE BiOS, 2018, San Francisco, California, United States

Abstract

Melanoma, developing from melanocytes, is the most serious type of skin cancer. Circulating melanoma cells, the prognosis marker for metastasis, are present in the circulation at the early stage. Thus, quantitative detection of rare circulating melanoma cells is essential for monitoring tumor metastasis and prognosis evaluation. Compared with in vitro assays, in vivo flow cytometry is able to identify circulating tumor cells without drawing blood. Here, we built in vivo photoacoustic flow cytometry based on the high absorption coefficient of melanoma cells, which is applied to labelfree counting of circulating melanoma cells in tumor-bearing mice.

Citation Download Citation

Quanyu Zhou, Ping Yang, Qiyan Wang, Kai Pang, Hui Zhou, Hao He, and Xunbin Wei "Label-free counting of circulating cells by in vivo photoacoustic flow cytometry", Proc. SPIE 10495, Biophotonics and Immune Responses XIII, 104950T (19 February 2018); https://doi.org/10.1117/12.2289216

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