Effect of near infrared lasers and glycated chitosan on myofibroblast differentiation and contraction

Christiana C. Obioma; Elivia Layton; Khue Tu Doan; Gang Xu; Feifan Zhou; Wei R. Chen; Melville B. Vaughan

doi:10.1117/12.2292250

19 February 2018 Effect of near infrared lasers and glycated chitosan on myofibroblast differentiation and contraction

Christiana C. Obioma, Elivia Layton, Khue Tu Doan, Gang Xu, Feifan Zhou, Wei R. Chen, Melville B. Vaughan

Proceedings Volume 10495, Biophotonics and Immune Responses XIII; 1049518 (2018) https://doi.org/10.1117/12.2292250
Event: SPIE BiOS, 2018, San Francisco, California, United States

Abstract

The ability to modulate myofibroblast phenotype will have an impact in wound healing, aging, cancer, and Dupuytren’s contracture development. Our project focused on investigating the effect of 808 nm laser wavelength on the presence of myofibroblast and contraction using anchored delayed release collagen lattice models. Fibroblast cells were cultured and suspended in collagen lattices in the presence or absence of glycated chitosan (GC) to mimic a wound healing environment in-vitro. The cells were allowed to generate maximum tension for 6 days followed by laser light stimulation. The total number of cells and presence of fibroblast proliferation and differentiation into myofibroblasts was determined using immunostaining followed by fluorescent microscope photography. Lattice tension generation was assessed by releasing the lattices from their anchorage and measuring their diameters. Control lattices contracted more than GC lattices. Myofibroblast differentiation was slightly higher in the control group. Repeats of the experiment are needed to confirm our results to determine if near infra-red laser radiation can be applied to treat open wounds and study tumor stromas.

Citation Download Citation

Christiana C. Obioma, Elivia Layton, Khue Tu Doan, Gang Xu, Feifan Zhou, Wei R. Chen, and Melville B. Vaughan "Effect of near infrared lasers and glycated chitosan on myofibroblast differentiation and contraction", Proc. SPIE 10495, Biophotonics and Immune Responses XIII, 1049518 (19 February 2018); https://doi.org/10.1117/12.2292250

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