Cross-level sampling for bandwidth effective time-domain fluorescence lifetime imaging implementation

Elvis Duran; Sebastian Hoyos; Javier Jo

doi:10.1117/12.2287478

2 March 2018 Cross-level sampling for bandwidth effective time-domain fluorescence lifetime imaging implementation

Elvis Duran, Sebastian Hoyos, Javier Jo

Author Affiliations +

Proceedings Volume 10499, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXV; 104991N (2018) https://doi.org/10.1117/12.2287478
Event: SPIE BiOS, 2018, San Francisco, California, United States

Abstract

The implementation of time domain fluorescence lifetime imaging (TD-FLIM) requires high cost bandwidth electronics and large storage capacity. A cross-level sampling technique for TD-FLIM is proposed. A simulation of this sampling technique was implemented using synthetic FLIM data. FLIM images were synthetically generated at different noise levels using a wide range of lifetimes. Each of the images was resampled using a cross-level approach and the lifetime maps were computed. Simulation results displayed strong correlation between the lifetime maps of the original and resampled images, suggesting that this sampling method could be adopted to reduce bandwidth and data transfer/ storage requirements.

Citation Download Citation

Elvis Duran, Sebastian Hoyos, and Javier Jo "Cross-level sampling for bandwidth effective time-domain fluorescence lifetime imaging implementation", Proc. SPIE 10499, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXV, 104991N (2 March 2018); https://doi.org/10.1117/12.2287478

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