Multi-spectral digital holographic microscopy for enhanced quantitative phase imaging of living cells

Björn Kemper; Lena Kastl; Jürgen Schnekenburger; Steffi Ketelhut

doi:10.1117/12.2291071

23 February 2018 Multi-spectral digital holographic microscopy for enhanced quantitative phase imaging of living cells

Björn Kemper, Lena Kastl, Jürgen Schnekenburger, Steffi Ketelhut

Proceedings Volume 10503, Quantitative Phase Imaging IV; 1050313 (2018) https://doi.org/10.1117/12.2291071
Event: SPIE BiOS, 2018, San Francisco, California, United States

Abstract

Main restrictions of using laser light in digital holographic microscopy (DHM) are coherence induced noise and parasitic reflections in the experimental setup which limit resolution and measurement accuracy. We explored, if coherence properties of partial coherent light sources can be generated synthetically utilizing spectrally tunable lasers. The concept of the method is demonstrated by label-free quantitative phase imaging of living pancreatic tumor cells and utilizing an experimental configuration including a commercial microscope and a laser source with a broad tunable spectral range of more than 200 nm.

Citation Download Citation

Björn Kemper, Lena Kastl, Jürgen Schnekenburger, and Steffi Ketelhut "Multi-spectral digital holographic microscopy for enhanced quantitative phase imaging of living cells", Proc. SPIE 10503, Quantitative Phase Imaging IV, 1050313 (23 February 2018); https://doi.org/10.1117/12.2291071

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