Phase shifting white light interferometry using colour CCD for optical metrology and bio-imaging applications

Paul Kumar Upputuri; Manojit Pramanik

doi:10.1117/12.2287090

23 February 2018 Phase shifting white light interferometry using colour CCD for optical metrology and bio-imaging applications

Paul Kumar Upputuri, Manojit Pramanik

Author Affiliations +

Proceedings Volume 10503, Quantitative Phase Imaging IV; 105032E (2018) https://doi.org/10.1117/12.2287090
Event: SPIE BiOS, 2018, San Francisco, California, United States

Abstract

Phase shifting white light interferometry (PSWLI) has been widely used for optical metrology applications because of their precision, reliability, and versatility. White light interferometry using monochrome CCD makes the measurement process slow for metrology applications. WLI integrated with Red-Green-Blue (RGB) CCD camera is finding imaging applications in the fields optical metrology and bio-imaging. Wavelength dependent refractive index profiles of biological samples were computed from colour white light interferograms. In recent years, whole-filed refractive index profiles of red blood cells (RBCs), onion skin, fish cornea, etc. were measured from RGB interferograms. In this paper, we discuss the bio-imaging applications of colour CCD based white light interferometry. The approach makes the measurement faster, easier, cost-effective, and even dynamic by using single fringe analysis methods, for industrial applications.

Citation Download Citation

Paul Kumar Upputuri and Manojit Pramanik "Phase shifting white light interferometry using colour CCD for optical metrology and bio-imaging applications", Proc. SPIE 10503, Quantitative Phase Imaging IV, 105032E (23 February 2018); https://doi.org/10.1117/12.2287090

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