CONFERENCE PROCEEDINGS
Advanced Search
Home > Proceedings > Volume 10506 > Article
Presentation
15 March 2018 Nanoscale light confinement and fluorescence excitation using plasmonic metal nanostructures (Conference Presentation)
Kiheung Kim, Hongki Lee, Donghyun Kim
Author Affiliations +
Proceedings Volume 10506, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XV; 105060O (2018) https://doi.org/10.1117/12.2291420
Event: SPIE BiOS, 2018, San Francisco, California, United States
Abstract
It is required to reduce the excitation volume of fluorescence with enhanced field intensity to apply fluorescence correlation spectroscopy (FCS) technique to the investigation of biological molecules. Field localization induced by plasmonic nanostructures enables measurements of molecular dynamics under high concentration exhibiting high signal-to-noise (SNR) ratio. To achieve this goal, we have investigated the feasibility of plasmonic monomer and dimer nanostructures for FCS techniques. We have studied field enhancement and localization induced by different gold monomer arrays whose shapes were circle, rhombus and triangle and also gold dimer arrays which had a gap of 10 nm. These plasmnoic nanostructures were considered to be on the gold film and glass substrate with chrome adhesion layer. We could shift the peak wavelength of field enhancement by changing the dimensions of nanostructures to spectrally overlap the field enhancement to excitation and emission spectrum of fluorophores. In the case of dimer configuration having a 90-nm diameter and a 20-nm height, we have induced the near-field localization with a light source at 671 nm whose dimension was 18×6×6 nm^3 with an enhanced field intensity by 500 times in comparison with a incident light. The field distribution was analyzed numerically and experimentally using finite-difference-time-domain method and near-field scanning optical microscope. We could measure the diffusion coefficients of 50-nm fluorescent beads with improved SNR which was found to be 44.6×10-14 m^2/s. Results would include the diffusion mapping of fluorescence molecules using imaging FCS technique to show the plasmnoic nanostructures is applicable to nanoscale FCS for study of molecular biology.
Conference Presentation
© (2018) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Citation Download Citation
Kiheung Kim, Hongki Lee, and Donghyun Kim "Nanoscale light confinement and fluorescence excitation using plasmonic metal nanostructures (Conference Presentation)", Proc. SPIE 10506, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XV, 105060O (15 March 2018); https://doi.org/10.1117/12.2291420
ACCESS THE FULL ARTICLE
ORGANIZATIONAL
Sign in with credentials provided by your organization.
INSTITUTIONAL
Select your institution to access the SPIE Digital Library.
SELECT YOUR INSTITUTION
PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.
PERSONAL SIGN IN
No SPIE Account? Create one
PURCHASE THIS CONTENT
SUBSCRIBE TO DIGITAL LIBRARY
50 downloads per 1-year subscription
Members: $195
Non-members: $335 ADD TO CART
25 downloads per 1 - year subscription
Members: $145
Non-members: $250 ADD TO CART
PURCHASE SINGLE ARTICLE
Includes PDF, HTML & Video, when available
Members: $17.00
Non-members: $21.00 ADD TO CART
PROCEEDINGS
 PRESENTATION
WATCH
PRESENTATION SAVE TO MY LIBRARY
GET CITATION
Advertisement
Advertisement
KEYWORDS
Nanostructures
Fluorescence correlation spectroscopy
Gold
Luminescence
Near field scanning optical microscopy
Plasmonics
Signal to noise ratio
RELATED CONTENT
Subscribe to Digital Library
Receive Erratum Email Alert
Back to Top