Dual-radiolabeled nanoparticle probes for depth-independent in vivo imaging of enzyme activation

Kvar C. L. Black; Mingzhou Zhou; Pinaki Sarder; Maryna Kuchuk; Amal Y. Al-Yasiri; Sean P. Gunsten; Kexian Liang; Heather M. Hennkens; Walter J. Akers; Richard Laforest; Steven L. Brody; Cathy S. Cutler; Samuel Achilefu

doi:10.1117/12.2301033

20 February 2018 Dual-radiolabeled nanoparticle probes for depth-independent in vivo imaging of enzyme activation

Kvar C. L. Black, Mingzhou Zhou, Pinaki Sarder, Maryna Kuchuk, Amal Y. Al-Yasiri, Sean P. Gunsten, Kexian Liang, Heather M. Hennkens, Walter J. Akers, Richard Laforest, Steven L. Brody, Cathy S. Cutler, Samuel Achilefu

Author Affiliations +

Proceedings Volume 10508, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications X; 1050805 (2018) https://doi.org/10.1117/12.2301033
Event: SPIE BiOS, 2018, San Francisco, California, United States

Abstract

Quantitative and noninvasive measurement of protease activities has remained an imaging challenge in deep tissues such as the lungs. Here, we designed a dual-radiolabeled probe for reporting the activities of proteases such as matrix metalloproteinases (MMPs) with multispectral single photon emission computed tomography (SPECT) imaging. A gold nanoparticle (NP) was radiolabeled with ¹²⁵I and ¹¹¹In and functionalized with an MMP9-cleavable peptide to form a multispectral SPECT imaging contrast agent. In another design, incorporation of ¹⁹⁹Au radionuclide into the metal crystal structure of gold NPs provided a superior and stable reference signal in lungs, and ¹¹¹In was linked to the NP surface via a protease-cleavable substrate, which can serve as an enzyme activity reporter. This work reveals strategies to correlate protease activities with diverse pathologies in a tissue-depth independent manner.

Citation Download Citation

Kvar C. L. Black, Mingzhou Zhou, Pinaki Sarder, Maryna Kuchuk, Amal Y. Al-Yasiri, Sean P. Gunsten, Kexian Liang, Heather M. Hennkens, Walter J. Akers, Richard Laforest, Steven L. Brody, Cathy S. Cutler, and Samuel Achilefu "Dual-radiolabeled nanoparticle probes for depth-independent in vivo imaging of enzyme activation", Proc. SPIE 10508, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications X, 1050805 (20 February 2018); https://doi.org/10.1117/12.2301033

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