Analytical Applications Of High-Resolution Molecular Fluorescence Spectroscopy In Low Temperature Solid Matrices

J. W. Hofstraat; W. J. M. van Zeijl; F. Smedes; F. Ariese; C. Gooijer; N. H. Velthorst; R. Locher; A. Renn; U. P. Wild

doi:10.1117/12.951551

17 May 1989 Analytical Applications Of High-Resolution Molecular Fluorescence Spectroscopy In Low Temperature Solid Matrices

J. W. Hofstraat, W. J. M. van Zeijl, F. Smedes, F. Ariese, C. Gooijer, N. H. Velthorst, R. Locher, A. Renn, U. P. Wild

Author Affiliations +

Proceedings Volume 1054, Fluorescence Detection III; (1989) https://doi.org/10.1117/12.951551
Event: OE/LASE '89, 1989, Los Angeles, CA, United States

Abstract

High-resolution fluorescence spectroscopy may be used to obtain highly specific, vibrationally resolved spectral signatures of molecules. Two techniques are presented that both make use of low temperature, solid matrices. In Shpol'skii spectroscopy highly resolved spectra are obtained by employing n-alkanes as solvents that form neat crystalline matrices at low temperatures in which the guest molecules occupy well defined substitutional sites. Fluorescence line-narrowing spectroscopy is based on the application of selective (mostly laser-) excitation of the guest molecules. Principles and analytical applications of both techniques will be discussed. Specific attention will be paid to the determination of pyrene in bird meat by means of Shpol'skii spectroscopy and to the possibilities of applying two-dimensional fluorescence line-narrowing spectroscopy.

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J. W. Hofstraat, W. J. M. van Zeijl, F. Smedes, F. Ariese, C. Gooijer, N. H. Velthorst, R. Locher, A. Renn, and U. P. Wild "Analytical Applications Of High-Resolution Molecular Fluorescence Spectroscopy In Low Temperature Solid Matrices", Proc. SPIE 1054, Fluorescence Detection III, (17 May 1989); https://doi.org/10.1117/12.951551

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