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22 February 2018 Use of digital micromirror devices as dynamic pinhole arrays for adaptive confocal fluorescence microscopy
Paolo Pozzi, Dean Wilding, Oleg Soloviev, Gleb Vdovin, Michel Verhaegen
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Proceedings Volume 10546, Emerging Digital Micromirror Device Based Systems and Applications X; 105460D (2018) https://doi.org/10.1117/12.2288436
Event: SPIE OPTO, 2018, San Francisco, California, United States
Abstract
In this work, we present a new confocal laser scanning microscope capable to perform sensorless wavefront optimization in real time. The device is a parallelized laser scanning microscope in which the excitation light is structured in a lattice of spots by a spatial light modulator, while a deformable mirror provides aberration correction and scanning. A binary DMD is positioned in an image plane of the detection optical path, acting as a dynamic array of reflective confocal pinholes, images by a high performance cmos camera. A second camera detects images of the light rejected by the pinholes for sensorless aberration correction.
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Paolo Pozzi, Dean Wilding, Oleg Soloviev, Gleb Vdovin, and Michel Verhaegen "Use of digital micromirror devices as dynamic pinhole arrays for adaptive confocal fluorescence microscopy", Proc. SPIE 10546, Emerging Digital Micromirror Device Based Systems and Applications X, 105460D (22 February 2018); https://doi.org/10.1117/12.2288436
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KEYWORDS
Digital micromirror devices
Confocal microscopy
Microscopes
Adaptive optics
Deformable mirrors
Microscopy
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