Yeast concentration analysis by using the portable microscope based on the fiber-optic array

Weiming Wang; Long Li; Yan Yu; Hui Huang

doi:10.1117/12.2295921

23 March 2018 Yeast concentration analysis by using the portable microscope based on the fiber-optic array

Weiming Wang, Long Li, Yan Yu, Hui Huang

Proceedings Volume 10597, Nano-, Bio-, Info-Tech Sensors, and 3D Systems II; 105971C (2018) https://doi.org/10.1117/12.2295921
Event: SPIE Smart Structures and Materials + Nondestructive Evaluation and Health Monitoring, 2018, Denver, Colorado, United States

Abstract

Yeast cells are always used in the biofuel, alcoholic and baking industries. Real-time monitoring of the yeast concentration without any algorithm processing is significant to master the yeast cells surviving condition. Here we demonstrate a new method to monitor the yeast cell concentration using our previous developed microscope—portable microscopy based on fiber-optic array (FOA). The sample is illuminated by a broadband LED and a light diffuser is used to create uniform illumination over the entire field-of-view (FOV). The image pre-processing including the denoising and binarization are used for the images optimization to increase the signal-to-noise ratio (SNR). Automated counting of the yeast cells is performed on a computer using ImageJ. The paper provides a new method for the counting of the yeast cells.

Citation Download Citation

Weiming Wang, Long Li, Yan Yu, and Hui Huang "Yeast concentration analysis by using the portable microscope based on the fiber-optic array", Proc. SPIE 10597, Nano-, Bio-, Info-Tech Sensors, and 3D Systems II, 105971C (23 March 2018); https://doi.org/10.1117/12.2295921

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