Förster resonance energy transfer between quantum dots and dye immobilized in biopolymer particles

M. A. Gerasimova; N. V. Slyusarenko; E. A. Slyusareva

doi:10.1117/12.2303488

16 April 2018 Förster resonance energy transfer between quantum dots and dye immobilized in biopolymer particles

M. A. Gerasimova, N. V. Slyusarenko, E. A. Slyusareva

Proceedings Volume 10614, International Conference on Atomic and Molecular Pulsed Lasers XIII; 106140E (2018) https://doi.org/10.1117/12.2303488
Event: XIII International Conference on Atomic and Molecular Pulsed Lasers, 2017, Tomsk, Russia

Abstract

We used high adsorption properties of the cationic biopolymer chitosan to synthesize colloidal polymer particles (average size about 0.3 μm) with immobilized CdTe quantum dots (QDs) and organic dye (erythrosin B). A high local concentration of fluorophores bound to the particles (about 10^–3 M), as well as a wide overlap of their optical spectra result in an efficient (up to 80%) Förster resonance energy transfer (FRET) from QDs ensembles to dye molecules. The FRET was registered by both steady-state (quenching of the donor and enhancement of the acceptor fluorescence) and time-resolved methods (decreasing of donor lifetime). The dependence of the transfer efficiency on acceptor concentration was analyzed within the scope of the Förster theory extended for the case of multiple energy transfer configuration. The average distances between the donor and acceptor as well as local concentration of fluorophores within particles were determined. It was demonstrated that the synthesized particles can be used as FRET-based sensitive probes for inter-fluorophore distance calculation within the range of 4 ÷ 9 nm.

Citation Download Citation

M. A. Gerasimova, N. V. Slyusarenko, and E. A. Slyusareva "Förster resonance energy transfer between quantum dots and dye immobilized in biopolymer particles", Proc. SPIE 10614, International Conference on Atomic and Molecular Pulsed Lasers XIII, 106140E (16 April 2018); https://doi.org/10.1117/12.2303488

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