ZnO nanoflakes-based mediator free flexible biosensors for the selective detection of  ethylglucuronide (EtG) and lactate

Fahmida Alam; Ahmed Hasnain Jalal; Raju Sinha; Yogeswaran Umasankar; Shekhar Bhansali; Nezih Pala

doi:10.1117/12.2306001

8 May 2018 ZnO nanoflakes-based mediator free flexible biosensors for the selective detection of ethylglucuronide (EtG) and lactate

Fahmida Alam, Ahmed Hasnain Jalal, Raju Sinha, Yogeswaran Umasankar, Shekhar Bhansali, Nezih Pala

Author Affiliations +

Proceedings Volume 10639, Micro- and Nanotechnology Sensors, Systems, and Applications X; 106392O (2018) https://doi.org/10.1117/12.2306001
Event: SPIE Defense + Security, 2018, Orlando, FL, United States

Abstract

We report on highly sensitive and flexible biosensors for noninvasive lactate and alcohol monitoring in human perspiration based on zinc oxide (ZnO) nanostructures that does not require linker layer for surface functionalization due to the high isoelectric point of ZnO. Towards fabrication of the biosensors, two-dimensional (2D) ZnO nanoflakes (NFs) were synthesized on flexible polyethylene terephthalate (PET) substrates employing single step sonochemical method after which lactate oxidase (LOx) and anti-body for ethyl glucuronide (EtG)-a metabolite of ethanol were immobilized atop without a linker layer. The cyclic voltammetry (CV) measurements in the concentration range of 10pM-10μM for lactate and 4.5 μM-0.45 M for EtG yielded minimum limit of detection of 10 pM and 4.5 μM, respectively for the electrode area of 0.5 × 0.5 cm². Moreover, lactate sensor with ZnO NF electrodes demonstrated four times higher sensitivity compared to the ones with gold electrode that required DTSP linker layer for surface functionalization. High isoelectric point allows a direct, stable pathway for rapid electron transport without any mediator when an analyte is immobilized on NFs and improves electron transfer rate.

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Fahmida Alam, Ahmed Hasnain Jalal, Raju Sinha, Yogeswaran Umasankar, Shekhar Bhansali, and Nezih Pala "ZnO nanoflakes-based mediator free flexible biosensors for the selective detection of ethylglucuronide (EtG) and lactate", Proc. SPIE 10639, Micro- and Nanotechnology Sensors, Systems, and Applications X, 106392O (8 May 2018); https://doi.org/10.1117/12.2306001

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