Presentation + Paper
24 May 2018 Residual flatness error correction in three-dimensional imaging confocal microscopes
Author Affiliations +
Abstract
Imaging Confocal Microscopes (ICM) are highly used for the assessment of three-dimensional measurement of technical surfaces. The benefit of an ICM in comparison to an interferometer is the use of high numerical aperture microscope objectives, which allows retrieving signal from high slope regions of a surface. When measuring a flat sample, such as a high-quality mirror, all ICM’s show a complex shape of low frequencies instead of a uniform flat result. Such shape, obtained from a λ/10, Sa < 0.5 nm calibration mirror is used as a reference for being subtracted from all the measurements, according to ISO 25178-607. This is true and valid only for those surfaces that have small slopes. When measuring surfaces with varying local slopes or tilted with respect to the calibration, the flatness error calibration is no longer valid, leaving what is called the residual flatness error.

In this paper we show that the residual flatness error on a reference sphere measured with a 10X can make the measurement of the radius to have up to 10% error. We analyzed the sources that generate this effect and proposed a method to correct it: we measured a tilted mirror with several angles and characterized the flatness error as a function of the distance to the optical axis, and the tilt angle. New measurements take into account such characterization by assessing the local slopes. We tested the method on calibrated reference spheres and proved to provide correct measurements. We also analyzed this behavior in Laser Scan as well on Microdisplay Scan confocal microscopes.
Conference Presentation
© (2018) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Carlos Bermudez, André Felgner, Pol Martinez, Aitor Matilla, Cristina Cadevall, and Roger Artigas "Residual flatness error correction in three-dimensional imaging confocal microscopes", Proc. SPIE 10678, Optical Micro- and Nanometrology VII, 106780M (24 May 2018); https://doi.org/10.1117/12.2306903
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CITATIONS
Cited by 1 scholarly publication.
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KEYWORDS
Microscopes

Calibration

Confocal microscopy

3D image processing

Monochromatic aberrations

Cameras

Error analysis

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