Dry mass and average phase shift dynamics in HeLa cells subjected to low-dose photodynamic treatment

A. V. Belashov; A. A. Zhikhoreva; T. N. Belyaeva; E. S. Kornilova; A. V. Salova; I. V. Semenova; O. S. Vasyutinskii

doi:10.1117/12.2306810

17 May 2018 Dry mass and average phase shift dynamics in HeLa cells subjected to low-dose photodynamic treatment

A. V. Belashov, A. A. Zhikhoreva, T. N. Belyaeva, E. S. Kornilova, A. V. Salova, I. V. Semenova, O. S. Vasyutinskii

Proceedings Volume 10685, Biophotonics: Photonic Solutions for Better Health Care VI; 1068505 (2018) https://doi.org/10.1117/12.2306810
Event: SPIE Photonics Europe, 2018, Strasbourg, France

Abstract

The paper presents results on the response of living HeLa cells in vitro to low-dose photodynamic treatment with Radachlorin photosensitizer. Quantitative monitoring of variations of optical and morphological parameters of cells was performed by means of digital holographic microscopy and assisted with observations in confocal fluorescent microscope. The statistical analysis of the results obtained demonstrated significant morphological changes of cells along with invariable dry mass. The AO/EB standard test validated cell membrane integrity and demonstrated cells rounding and membrane blebbing. These data allow us to assume apoptosis as a major pathway of cell death activated in our experimental conditions.

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Citation Download Citation

A. V. Belashov, A. A. Zhikhoreva, T. N. Belyaeva, E. S. Kornilova, A. V. Salova, I. V. Semenova, and O. S. Vasyutinskii "Dry mass and average phase shift dynamics in HeLa cells subjected to low-dose photodynamic treatment", Proc. SPIE 10685, Biophotonics: Photonic Solutions for Better Health Care VI, 1068505 (17 May 2018); https://doi.org/10.1117/12.2306810

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