Isotropic quantitative differential phase contrast microscopic imaging

Yu-Zi Lin; Hsi-Hsun Chen; Kuang-Yuh Huang; Yuan Luo

doi:10.1117/12.2316751

24 April 2018 Isotropic quantitative differential phase contrast microscopic imaging

Yu-Zi Lin, Hsi-Hsun Chen, Kuang-Yuh Huang, Yuan Luo

Proceedings Volume 10711, Biomedical Imaging and Sensing Conference; 107110R (2018) https://doi.org/10.1117/12.2316751
Event: SPIE Structured Light, 2018, Yokohama, Japan

Abstract

Differential phase contrast microscopic (DPCM) imaging is a popular methodology to recover quantitative phase image of thin transparent sample under multi-axis intensity measurements. To improve the accuracy and stability of phase recovery in conventional DPCM imaging effectively, we propose a new illumination method to achieve isotropic differential phase contrast microscopic (iDPCM) imaging efficiently. Our iDPCM imaging is implemented with a partially coherent microscopy, and a programmable thin-film transistor panel to modulate the illumination pattern. We demonstrate our theoretical approach for iDPCM imaging and experimental results of quantitative phase image of a microlens array and unstained live human cells.

Citation Download Citation

Yu-Zi Lin, Hsi-Hsun Chen, Kuang-Yuh Huang, and Yuan Luo "Isotropic quantitative differential phase contrast microscopic imaging", Proc. SPIE 10711, Biomedical Imaging and Sensing Conference, 107110R (24 April 2018); https://doi.org/10.1117/12.2316751

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