Application of laser scanning speckle-microscopy for high-resolution express diagnostics of chlamydial infection

Sergey Ulyanov; Olga Larionova; Onega Ulianova; Sergey Zaitsev; Yury Saltykov; Tatiana Polyanina; Anna Lyapina; Nadezhda Filonova; Irina Subbotina; Irina Kalduzova; Sergey Utz; Yulia Moiseeva; Valentina Feodorova

doi:10.1117/12.2314703

26 April 2018 Application of laser scanning speckle-microscopy for high-resolution express diagnostics of chlamydial infection

Sergey Ulyanov, Olga Larionova, Onega Ulianova, Sergey Zaitsev, Yury Saltykov, Tatiana Polyanina, Anna Lyapina, Nadezhda Filonova, Irina Subbotina, Irina Kalduzova, Sergey Utz, Yulia Moiseeva, Valentina Feodorova

Author Affiliations +

Proceedings Volume 10716, Saratov Fall Meeting 2017: Optical Technologies in Biophysics and Medicine XIX; 107160N (2018) https://doi.org/10.1117/12.2314703
Event: Saratov Fall Meeting 2017, 2017, Saratov, Russian Federation

Abstract

Method of speckle-microscopy has been adapted to the problem of detection of Chlamydia trachomatis microbial cells in clinical samples. Prototype of laser scanning speckle-microscope has been designed. Spatial resolution and output characteristics of this microscope have been analyzed for the case of scanning of C. trachomatis bacteria inclusions – Elementary Bodies (EBs) inside the human cells, fixed on the glass. It has been demonstrated, that presence of C. trachomatis microbial cells in the sample can be easily detected using speckle microscopy.

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Sergey Ulyanov, Olga Larionova, Onega Ulianova, Sergey Zaitsev, Yury Saltykov, Tatiana Polyanina, Anna Lyapina, Nadezhda Filonova, Irina Subbotina, Irina Kalduzova, Sergey Utz, Yulia Moiseeva, and Valentina Feodorova "Application of laser scanning speckle-microscopy for high-resolution express diagnostics of chlamydial infection", Proc. SPIE 10716, Saratov Fall Meeting 2017: Optical Technologies in Biophysics and Medicine XIX, 107160N (26 April 2018); https://doi.org/10.1117/12.2314703

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