Detection of oligomerization of Epstein-Barr virus oncoprotein LMP1 by FRET-based probes

Zhiwei Wu; Qing Ye; Jing Li; Yimei Huang; Yating Lin; Mengmeng Zheng; Shusen Xie; Juqiang Lin

doi:10.1117/12.2500650

23 October 2018 Detection of oligomerization of Epstein-Barr virus oncoprotein LMP1 by FRET-based probes

Zhiwei Wu, Qing Ye, Jing Li, Yimei Huang, Yating Lin, Mengmeng Zheng, Shusen Xie, Juqiang Lin

Proceedings Volume 10820, Optics in Health Care and Biomedical Optics VIII; 108201T (2018) https://doi.org/10.1117/12.2500650
Event: SPIE/COS Photonics Asia, 2018, Beijing, China

Abstract

In this study, two FRET-based probes are constructed to research oligomerization of Epstein-Barr virus Oncoprotein LMP1 in live cells. The images of wide-field fluorescence microscopy display that the majority of two LMP1-associated probes co-localized in internal perinuclear membranes. Furthermore, the fluorescence spectra of single cell co-expressed two probes indicated that the ratio of two emission peaks is around one, and the fluorescence spectra changed insignificantly during an hour observation. These findings indicated that LMP1/LMP1 interacted stably in live cells.

Citation Download Citation

Zhiwei Wu, Qing Ye, Jing Li, Yimei Huang, Yating Lin, Mengmeng Zheng, Shusen Xie, and Juqiang Lin "Detection of oligomerization of Epstein-Barr virus oncoprotein LMP1 by FRET-based probes", Proc. SPIE 10820, Optics in Health Care and Biomedical Optics VIII, 108201T (23 October 2018); https://doi.org/10.1117/12.2500650

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KEYWORDS

Luminescence

Fluorescence resonance energy transfer

Proteins

Microscopy

Spectrographs

Fluorescent proteins

Image filtering

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