Noninvasive assessment of light scattering and hemoglobin in cutaneous two-stage chemical carcinogenesis of mice based on multispectral diffuse reflectance images

Md Abdul Wares; Naoki Tobita; Masaki Inaba; Satoko Kawauchi; Shunichi Sato; Izumi Nishidate

doi:10.1117/12.2509793

26 February 2019 Noninvasive assessment of light scattering and hemoglobin in cutaneous two-stage chemical carcinogenesis of mice based on multispectral diffuse reflectance images

Md Abdul Wares, Naoki Tobita, Masaki Inaba, Satoko Kawauchi, Shunichi Sato, Izumi Nishidate

Author Affiliations +

Proceedings Volume 10851, Photonics in Dermatology and Plastic Surgery 2019; 1085113 (2019) https://doi.org/10.1117/12.2509793
Event: SPIE BiOS, 2019, San Francisco, California, United States

Abstract

Due to the topographical location and extensive size, skin encounters high dose of clastogen those cause cancer which can be cured, if diagnosed at the early stage. While visual inspection, histopathological study, bio-sensing, dermoscopy exhibit some limitations, noninvasive optical methods cater comfortable, early and precise diagnosis. In this research, we investigated a multispectral imaging method based on the diffuse reflectance spectroscopy (DRS) to estimate spatiotemporal changes in the light scattering and hemodynamic parameters in mice during cutaneous two-stage chemical carcinogenesis. In this method, Monte Carlo simulation-based empirical formulas assisted in the extraction of the light scattering power b, total hemoglobin concentration C_th, and tissue oxygen saturation StO₂ in the skin. In laboratory environment, we induced mice skin cancer by 7,12-dimethylbenz[a]anthracene (DMBA) and 12-Otetradecanoylphorbol-13-acetate (TPA) and monitored the changes in the cutaneous tissue at a particular interval through capturing multispectral diffuse reflectance images and analyzing over the period of initiation, promotion and progression. The results displayed the decrease in b and increases in both C_th and StO₂ in tumor regions. Significantly, we found that the inception of rapid changes in the scattering parameter is about one to two week(s) earlier than the hemoglobin concentration. On the other hand, at the advanced stage, we also found the blackish discoloration of the skin in the tip of the papilloma when it experienced necrosis, which corresponds to the regional decrease in StO₂ of some large papilloma.

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Md Abdul Wares, Naoki Tobita, Masaki Inaba, Satoko Kawauchi, Shunichi Sato, and Izumi Nishidate "Noninvasive assessment of light scattering and hemoglobin in cutaneous two-stage chemical carcinogenesis of mice based on multispectral diffuse reflectance images", Proc. SPIE 10851, Photonics in Dermatology and Plastic Surgery 2019, 1085113 (26 February 2019); https://doi.org/10.1117/12.2509793

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KEYWORDS

Skin

Tissues

Diffuse reflectance spectroscopy

Light scattering

Oxygen

Scattering

Multispectral imaging

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