Combined confocal and photoacoustic microscopy based on probe beam deflection technique

Robert Nuster; Rainer Hofmann-Wellenhof; Guenther Paltauf

doi:10.1117/12.2507502

27 February 2019 Combined confocal and photoacoustic microscopy based on probe beam deflection technique

Robert Nuster, Rainer Hofmann-Wellenhof, Guenther Paltauf

Proceedings Volume 10878, Photons Plus Ultrasound: Imaging and Sensing 2019; 108784O (2019) https://doi.org/10.1117/12.2507502
Event: SPIE BiOS, 2019, San Francisco, California, United States

Abstract

A device for acquiring co-registered reflectance confocal microscopy (RCM) and optical-resolution photoacoustic microscopy (OR-PAM) images is presented. It uses a pulsed laser for photoacoustic excitation and a continuous laser for the confocal mode, co-aligned, focused and scanned over planes within the object. The photoacoustic part uses a probe beam deflection method, where a continuous laser beam is bent in the refractive index gradient generated by the pressure wave. First images on a carbon microfiber phantom demonstrate the capability of generating co-registered images, where the RCM mode displays back-scattering contrast, while the OR-PAM mode creates images with optical absorption contrast. In the temporal signals of both methods, typical signatures of transient cavitation were visible in the carbon fiber experiment. A lateral resolution of the setup of about 1.6 μm and a noise equivalent pressure amplitude of 90 Pa for the probe beam deflection method were measured.

Citation Download Citation

Robert Nuster, Rainer Hofmann-Wellenhof, and Guenther Paltauf "Combined confocal and photoacoustic microscopy based on probe beam deflection technique", Proc. SPIE 10878, Photons Plus Ultrasound: Imaging and Sensing 2019, 108784O (27 February 2019); https://doi.org/10.1117/12.2507502

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