Altered stress granules biomechanics by ALS protein FUS revealed by background-deflection Brillouin microscopy (Conference Presentation)

Giuseppe Antonacci

doi:10.1117/12.2509477

4 March 2019 Altered stress granules biomechanics by ALS protein FUS revealed by background-deflection Brillouin microscopy (Conference Presentation)

Giuseppe Antonacci

Author Affiliations +

Proceedings Volume 10880, Optical Elastography and Tissue Biomechanics VI; 108800A (2019) https://doi.org/10.1117/12.2509477
Event: SPIE BiOS, 2019, San Francisco, California, United States

Abstract

Demand to reveal fundamental micro-mechanical properties is driven by a growing evidence that altered cellular processes in aging-associated disease environments are caused by a change in the regulating biomechanics. Unlike standard elastography techniques, Brillouin microscopy has shown great capabilities to non-invasively assess the biomechanics in the volume of biological samples, such as the lens cornea, atherosclerotic plaques and cells. Spectral contrast is key in Brillouin microscopy to optically probe biological systems, where the elastic Rayleigh scattering and specular reflection are orders of magnitude greater than the Brillouin signal. Here, we developed a noncontact and label-free imaging method, named background-deflection Brillouin (BDB) microscopy, to investigate the three-dimensional intracellular biomechanics at a sub-micron resolution. Our method exploits diffraction to achieve an unprecedented 10,000-fold enhancement in the spectral contrast of single-stage spectrometers, enabling the first direct biomechanical analysis on intracellular stress granules containing ALS mutant FUS protein in fixed cells. Our results provide insights on an aberrant liquid-to-solid phase transition observed in in-vitro reconstituted droplets of FUS protein, which has been recently proposed as a possible pathogenic mechanism for ALS.

Conference Presentation

Citation Download Citation

Giuseppe Antonacci "Altered stress granules biomechanics by ALS protein FUS revealed by background-deflection Brillouin microscopy (Conference Presentation)", Proc. SPIE 10880, Optical Elastography and Tissue Biomechanics VI, 108800A (4 March 2019); https://doi.org/10.1117/12.2509477

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